From XenWiki
(Difference between pages)
|
imported>Virgilio |
| Line 1: |
Line 1: |
| '''Antigen:''' E-cadherin
| | #REDIRECT [[fluorescent in-situs and FCIS (Vize lab)]] |
|
| |
| '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=12531593 cdh1]
| |
|
| |
| '''Epitope:''' 140 kDa xenopus E-cadherin
| |
|
| |
| '''Host Species/Isotype:''' mouse monoclonal IgG2b
| |
|
| |
| '''Antigen Species:''' xenopus
| |
|
| |
| '''Applications:''' IF
| |
|
| |
| '''Antibody Name/Catalog Number:''' [http://dshb.biology.uiowa.edu/cadherin-E- 5D3]
| |
|
| |
| '''Supplier:''' Developmental Systems Hybridoma Bank
| |
|
| |
| '''Comments:''' IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300
| |
|
| |
| '''Contributor:''' Zorn Lab and V Ponferrada
| |
|
| |
| '''Additional Information:''' MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work
| |
|
| |
| '''Reference(s):'''
| |
|
| |
|
| |
|
| |
| applications abbreviations:
| |
|
| |
| WB - Western blotting,
| |
|
| |
| IP - immunoprecipitation,
| |
|
| |
| IF - immunofluorescence,
| |
|
| |
| IHC - immunohistochemistry,
| |
|
| |
| EMSA - electromobility shift assay,
| |
|
| |
| ChIP - chromatin immunoprecipitation,
| |
|
| |
| FCM - flow cytometry
| |
Latest revision as of 13:26, 15 November 2011