Histone H3, phosphorylated - h3f3a - mitotic cells - from upstate and File:CGP54626.png: Difference between pages

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'''Antigen:''' phosporylated histone H3 (Ser10)


'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6042438 h3f3a]
'''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
'''Host Species/Isotype:'''
'''Antigen Species:''' human
'''Applications:''' IF, WB
'''Antibody Name/Catalog Number:''' [http://www.millipore.com/catalogue/item/06-570 Cat # 06-570]
'''Supplier:''' Upstate/Millipore
'''Comments:''' not sentitive to bleaching, works post in situ
'''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
'''Additional Information:'''
[[File:Ph3.jpg|option|400px]]
A Xenopus pronephros stained with atp1a1 to highlight the pronephros in green, and countersrtained with phospho-H# in red to map cells in metaphase
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry

Revision as of 11:22, 28 May 2015