Wholemount antibody staining (CSH Protocols) (Wallingford lab)

From XenWiki
Jump to navigation Jump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

There are currently several published protocols for this. We (Wallingford lab) have recently published a protocol in CSH Protocols:

http://cshprotocols.cshlp.org/cgi/content/full/2008/3/pdb.prot4957?maxtoshow=&hits=10&RESULTFORMAT=&fulltext=wallingford&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT

That protocol draws from the many excellent previous protcols including:


Robinson, C. and Guille, M. 1999. Immunohistochemistry of Xenopus embryos. Methods Mol. Biol. 127: 89–97.

Sive, H.L., Grainger, R.M., and Harland, R.M. 2000. Early development of Xenopus laevis: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

Becker, B.E and Gard, D.L. 2006. Visualization of the cytoskeleton in Xenopus oocytes and eggs by confocal immunofluorescence microscopy. Methods Mol. Biol. 322: 69–86.