12/101 - somites, skeletal muscle marker: Difference between revisions
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'''Antigen:''' | '''Antigen:''' homogenized tissue | ||
'''Epitope:''' | '''Xenbase Gene Page:''' | ||
'''Host Species/Isotype:''' | '''Epitope:''' | ||
'''Antigen Species:''' | '''Host Species/Isotype:''' mouse monoclonal IgG | ||
'''Applications:''' | '''Antigen Species:''' newt | ||
'''Antibody Name:''' | '''Applications:''' IF, WB | ||
'''Supplier:''' | '''Antibody Name/Catalog Number:''' [http://dshb.biology.uiowa.edu/skeletal-muscle-marker 12/101] | ||
'''Comments:''' | '''Supplier:''' Developmental Studies Hybridoma Bank | ||
'''Contributor:''' | '''Comments:''' WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit) | ||
'''Additional Information:''' | '''Contributor:''' Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article.do?method=display&articleId=40901] | ||
'''Reference(s):''' | '''Additional Information:''' | ||
applications abbreviations: | '''Reference(s):''' | ||
WB - Western blotting, | |||
IP - immunoprecipitation, | applications abbreviations: | ||
IF - immunofluorescence, | WB - Western blotting, | ||
IHC - immunohistochemistry, | IP - immunoprecipitation, | ||
EMSA - electromobility shift assay, | IF - immunofluorescence, | ||
ChIP - chromatin immunoprecipitation, | IHC - immunohistochemistry, | ||
FCM - flow cytometry | EMSA - electromobility shift assay, | ||
ChIP - chromatin immunoprecipitation, | |||
[[File:12101.jpg]] | FCM - flow cytometry | ||
Immunofluorescence using 12/101 | |||
[[File:12101+ATPase.jpg]] | |||
[[File:12101.jpg]] | |||
Immunofluorescence using 12/101 | |||
[[File:12101+ATPase.jpg]] | |||
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green. | Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green. |
Latest revision as of 12:58, 8 March 2011
Antigen: homogenized tissue
Xenbase Gene Page:
Epitope:
Host Species/Isotype: mouse monoclonal IgG
Antigen Species: newt
Applications: IF, WB
Antibody Name/Catalog Number: 12/101
Supplier: Developmental Studies Hybridoma Bank
Comments: WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)
Contributor: Generated and characterized by Kintner [1] and Brockes (1985) [2]
Additional Information:
Reference(s):
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry
Immunofluorescence using 12/101
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.