12/101 - somites, skeletal muscle marker: Difference between revisions
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Revision as of 13:16, 4 February 2011
Antigen: homogenized tissue
Epitope:
Host Species/Isotype: mouse monoclonal IgG
Antigen Species: newt
Applications: IF, WB
Antibody Name: 12/101
Supplier: Developmental Studies Hybridoma Bank
Comments: WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)
Contributor: Generated and characterized by Kintner [1] and Brockes (1985) [2]
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry
Immunofluorescence using 12/101
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.