12/101 - somites, skeletal muscle marker: Difference between revisions
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'''Applications:''' IF, WB | '''Applications:''' IF, WB | ||
'''Antibody Name:''' 12/101 | '''Antibody Name:''' 12/101 [http://dshb.biology.uiowa.edu/skeletal-muscle-marker] | ||
'''Supplier:''' Developmental Studies Hybridoma Bank | '''Supplier:''' Developmental Studies Hybridoma Bank | ||
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'''Contributor:''' Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article.do?method=display&articleId=40901] | '''Contributor:''' Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article.do?method=display&articleId=40901] | ||
'''Additional Information:''' | |||
'''Reference(s):''' | |||
applications abbreviations: | applications abbreviations: | ||
WB - Western blotting, | WB - Western blotting, | ||
IP - immunoprecipitation, | IP - immunoprecipitation, | ||
IF - immunofluorescence, | IF - immunofluorescence, | ||
IHC - immunohistochemistry, | IHC - immunohistochemistry, | ||
EMSA - electromobility shift assay, | EMSA - electromobility shift assay, | ||
ChIP - chromatin immunoprecipitation, | ChIP - chromatin immunoprecipitation, | ||
FCM - flow cytometry | FCM - flow cytometry | ||
[[File:12101.jpg]] | [[File:12101.jpg]] | ||
Immunofluorescence using 12/101 | Immunofluorescence using 12/101 | ||
[[File:12101+ATPase.jpg]] | [[File:12101+ATPase.jpg]] | ||
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green. | Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green. | ||
Revision as of 07:43, 8 March 2011
Antigen: homogenized tissue
Epitope:
Host Species/Isotype: mouse monoclonal IgG
Antigen Species: newt
Applications: IF, WB
Antibody Name: 12/101 [1]
Supplier: Developmental Studies Hybridoma Bank
Comments: WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)
Contributor: Generated and characterized by Kintner [2] and Brockes (1985) [3]
Additional Information:
Reference(s):
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry
Immunofluorescence using 12/101
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.