File:Example tail bud.jpg and High-salt solution for egg laying (Zorn lab/Ron Laskey): Difference between pages

Revision as of 09:24, 23 November 2011

Protocol submitted by VGP

RAL’s Ca++-free High Salt Barth: Egg Laying (8X)

___________________(1x solution)___________________________amounts to make 8X stock__________

_____________________________________________2 litres___________4 litres___________8 liters_________

NaCl_________________110 mM___________________102.85g___________205.71g___________308.55g

Tris base______________15 mM____________________29.075g___________58.15g____________87.23g

KCl____________________2 mM____________________2.385g_____________4.77g____________7.2g

Na2HPO4 (anhydrous)___0.5 mM____________________1.36g______________2.72g____________4.1g

NaHCO3________________2 mM____________________2.69g______________5.38g____________8.07g

Adjust pH to 7.6 with glacial acetic acid (approx. 25 mls for 4 litres)

MgSO4.7H2O____________1mM____________________7.89g______________3.945g___________11.84g

Make 8X stock, dilute to 1x solution in dH2O just before use. Maximum recommended storage: 8 weeks at 4oC.

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+Protocol submitted by VGP
+RAL’s Ca++-free High Salt Barth: Egg Laying (8X)
+___________________(1x solution)___________________________amounts to make 8X stock__________
+_____________________________________________2 litres___________4 litres___________8 liters_________
+NaCl_________________110 mM___________________102.85g___________205.71g___________308.55g
+Tris base______________15 mM____________________29.075g___________58.15g____________87.23g
+KCl____________________2 mM____________________2.385g_____________4.77g____________7.2g
+Na2HPO4 (anhydrous)___0.5 mM____________________1.36g______________2.72g____________4.1g
+NaHCO3________________2 mM____________________2.69g______________5.38g____________8.07g
+Adjust pH to 7.6 with glacial acetic acid (approx. 25 mls for 4 litres)
+MgSO4.7H2O____________1mM____________________7.89g______________3.945g___________11.84g
+Make 8X stock, dilute to 1x solution in dH2O just before use.  Maximum recommended storage: 8 weeks at 4oC.