XB-FEAT-5889668 and High-salt solution for egg laying (Zorn lab/Ron Laskey): Difference between pages

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=unc80=
Protocol submitted by VGP
This is the community wiki page for the gene ''unc80'' please feel free to add any information that is relevant to this gene that is not already captured elsewhere in Xenbase
 
 
RAL’s Ca++-free High Salt Barth: Egg Laying (8X)
 
 
___________________(1x solution)___________________________amounts to make 8X stock_________________
 
_______________________________________________2 litres___________4 litres____________8 liters_________
 
NaCl_________________110 mM___________________102.85g___________205.71g___________308.55g________
 
Tris base______________15 mM____________________29.075g___________58.15g____________87.23g________
 
KCl____________________2 mM____________________2.385g_____________4.77g____________7.2g________
 
Na2HPO4 (anhydrous)___0.5 mM____________________1.36g______________2.72g____________4.1g________
 
NaHCO3________________2 mM____________________2.69g______________5.38g____________8.07g________
 
:Adjust pH to 7.6 with glacial acetic acid (approx. 25 mls for 4 litres)
 
MgSO4.7H2O____________1mM____________________7.89g______________3.945g___________11.84g________
 
 
Make 8X stock, dilute to 1x solution in dH2O just before use.  Maximum recommended storage: 8 weeks at 4oC.

Revision as of 09:59, 23 November 2011

Protocol submitted by VGP


RAL’s Ca++-free High Salt Barth: Egg Laying (8X)


___________________(1x solution)___________________________amounts to make 8X stock_________________

_______________________________________________2 litres___________4 litres____________8 liters_________

NaCl_________________110 mM___________________102.85g___________205.71g___________308.55g________

Tris base______________15 mM____________________29.075g___________58.15g____________87.23g________

KCl____________________2 mM____________________2.385g_____________4.77g____________7.2g________

Na2HPO4 (anhydrous)___0.5 mM____________________1.36g______________2.72g____________4.1g________

NaHCO3________________2 mM____________________2.69g______________5.38g____________8.07g________

Adjust pH to 7.6 with glacial acetic acid (approx. 25 mls for 4 litres)

MgSO4.7H2O____________1mM____________________7.89g______________3.945g___________11.84g________


Make 8X stock, dilute to 1x solution in dH2O just before use. Maximum recommended storage: 8 weeks at 4oC.