XB-FEAT-5719931 and High-salt solution for egg laying (Zorn lab/Ron Laskey): Difference between pages

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=loc100496133=  
Protocol submitted by VGP
This is the community wiki page for the gene ''loc100496133'' please feel free to add any information that is relevant to this gene that is not already captured elsewhere in Xenbase
 
 
RAL’s Ca++-free High Salt Barth: Egg Laying (8X) (Ron Laskey)
 
Place ovulating females in 1X high salt buffer, 3 liters per female.
 
Eggs laid in the high salt buffer remain viable for fertilization and microinjection.
 
 
 
{|border="1" cellpadding="5" cellspacing="0" align="center"
|-
|
! scope="col" style="background:#efefef;" |(1x solution)
! colspan="3" style="background:#efefef;" |amounts to make 8X stock
|-
! scope="row" style="background:#efefef;" |
|style="background:#efefef;"|
|style="background:#efefef;"|2 litres
|style="background:#efefef;"|4 litres
|style="background:#efefef;"|8 litres
|-
! scope="row" style="background:#efefef;" |NaCl
|110 mM
|102.85g
|205.71g
|308.55g
|-
! scope="row" style="background:#efefef;" |Tris base
|15 mM
|29.075g
|58.15g
|87.23g
|-
! scope="row" style="background:#efefef;" |KCl
|2 mM
|2.385g
|4.77g
|7.2g
|-
! scope="row" style="background:#efefef;" |Na2HPO4 (anhydrous)
|0.5 mM
|1.36g
|2.72g
|4.1g
|-
! scope="row" style="background:#efefef;" |NaHCO3
|0.5 mM
|1.36g
|2.72g
|4.1g
|-
! scope="row" style="background:#efefef;" |
|colspan="4" style="background:#efefef;"|Adjust pH to 7.6 with glacial acetic acid (approx. 25 mls for 4 litres)
|-
! scope="row" style="background:#efefef;" |MgSO4.7H2O
|1mM
|7.89g
|3.945g
|11.84g
|-
|}
 
 
Make 8X stock, dilute to 1x solution in dH2O just before use.  Maximum recommended storage: 8 weeks at 4oC.

Latest revision as of 10:13, 7 December 2011

Protocol submitted by VGP


RAL’s Ca++-free High Salt Barth: Egg Laying (8X) (Ron Laskey)

Place ovulating females in 1X high salt buffer, 3 liters per female.

Eggs laid in the high salt buffer remain viable for fertilization and microinjection.


(1x solution) amounts to make 8X stock
2 litres 4 litres 8 litres
NaCl 110 mM 102.85g 205.71g 308.55g
Tris base 15 mM 29.075g 58.15g 87.23g
KCl 2 mM 2.385g 4.77g 7.2g
Na2HPO4 (anhydrous) 0.5 mM 1.36g 2.72g 4.1g
NaHCO3 0.5 mM 1.36g 2.72g 4.1g
Adjust pH to 7.6 with glacial acetic acid (approx. 25 mls for 4 litres)
MgSO4.7H2O 1mM 7.89g 3.945g 11.84g


Make 8X stock, dilute to 1x solution in dH2O just before use. Maximum recommended storage: 8 weeks at 4oC.