Smad1 - smad1 - from invitrogen and Forskolin: Difference between pages

Revision as of 13:45, 2 June 2015

Description

Activator of adenylyl cyclase.

Marketed as a dietary supplement/alternative medication.

"Cell-permeable diterpenoid that possesses anti-hypertensive, positive inotropic, and adenylyl cyclase activating properties. Many of its biological effects are due to its activation of adenylyl cyclase and the resulting increase in intracellular cAMP concentration.1 Forskolin effects calcium currents and inhibits MAP kinase."

- -Sigma product description

forskolin structure, photo from Sigma-Aldrich

Genes Affected

adenylyl cyclase

Suppliers

Usage Notes

50uM

References

>786 Xenbase articles contain a reference to Forskolin according to textpresso

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-'''Antigen:'''		human smad1
+==Description==
+Activator of adenylyl cyclase.
-'''Xenbase Gene Page:'''		[http://www.xenbase.org/gene/showgene.do?method=display&geneId=1018192 smad1]
+Marketed as a dietary supplement/alternative medication.
-'''Epitope:'''		internal synthetic peptide of human smad1
-'''Host Species/Isotype:'''		rabbit polyclonal
+"Cell-permeable diterpenoid that possesses anti-hypertensive, positive inotropic, and adenylyl cyclase activating properties. Many of its biological effects are due to its activation of adenylyl cyclase and the resulting increase in intracellular cAMP concentration.1 Forskolin effects calcium currents and inhibits MAP kinase."
+**-Sigma product description
-'''Antigen Species:'''		human
+[[File:forskolin.png|frame|right|forskolin structure, photo from Sigma-Aldrich]]
-'''Applications:'''		WB
-'''Antibody Name/Catalog Number:'''		[http://products.invitrogen.com/ivgn/product/385400?ICID=search-385400 rabbit anti-smad1 38-5400]
+==Genes Affected==
+*[http://www.xenbase.org/gene/searchGene.do?method=search&searchIn=0&searchValue=adenylate%20cyclase&searchType=0 adenylyl cyclase]
-'''Supplier:'''		invitrogen
+==Suppliers==
+*[http://www.sigmaaldrich.com/catalog/product/sigma/f6886?lang=en&region=US Sigma F6886]
+*[http://www.scbt.com/datasheet-3562-forskolin.html Santa Cruz sc-3562]
+*[http://www.tocris.com/dispprod.php?ItemId=2337#.VWeG9tLBzRY Tocris 1099]
-'''Comments:'''		WB: use at 1:1000 to 1:5000
+==Usage Notes==
+uM
-'''Contributor:'''		Zorn Lab
+==References==
+*[https://pubchem.ncbi.nlm.nih.gov/compound/47936 PubChem]
+*[http://www.xenbase.org/literature/article.do?method=display&articleId=11986 Kim et al., 1999]
-'''Additional Information:'''
+>786 Xenbase articles contain a reference to Forskolin according to [http://www.xenbase.org/cgi-bin/textpresso/xenopus/search textpresso]
+*[[Small Molecules for Xenopus Research|Back To Small Molecules Home Page]]
-'''Reference(s):'''
-applications abbreviations:
-WB - Western blotting,
-IP - immunoprecipitation,
-IF - immunofluorescence,
-IHC - immunohistochemistry,
-EMSA - electromobility shift assay,
-ChIP - chromatin immunoprecipitation,
-FCM - flow cytometry