Rfc5 and E-cadherin - cdh1: Difference between pages

Latest revision as of 09:03, 8 March 2011

Antigen: E-cadherin

Epitope: 140 kDa xenopus E-cadherin

Host Species/Isotype: mouse monoclonal IgG2b

Antigen Species: xenopus

Applications: IF

Antibody Name: 5D3 [1]

Supplier: Developmental Systems Hybridoma Bank

Comments: IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300

Contributor: Zorn Lab and V Ponferrada

Additional Information: MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work

Reference(s):

applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

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-#REDIRECT [[XB-FEAT-1016199]]
+'''Antigen:'''		E-cadherin
+'''Epitope:'''		140 kDa xenopus E-cadherin
+'''Host Species/Isotype:'''		mouse monoclonal IgG2b
+'''Antigen Species:'''		xenopus
+'''Applications:'''		IF
+'''Antibody Name:'''		5D3 [http://dshb.biology.uiowa.edu/cadherin-E-]
+'''Supplier:'''		Developmental Systems Hybridoma Bank
+'''Comments:'''		IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300
+'''Contributor:'''		Zorn Lab and V Ponferrada
+'''Additional Information:'''		MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work
+'''Reference(s):'''
+applications abbreviations:
+WB - Western blotting,
+IP - immunoprecipitation,
+IF - immunofluorescence,
+IHC - immunohistochemistry,
+EMSA - electromobility shift assay,
+ChIP - chromatin immunoprecipitation,
+FCM - flow cytometry