XB-ANAT-338 and E-cadherin - cdh1: Difference between pages

Latest revision as of 09:03, 8 March 2011

Antigen: E-cadherin

Epitope: 140 kDa xenopus E-cadherin

Host Species/Isotype: mouse monoclonal IgG2b

Antigen Species: xenopus

Applications: IF

Antibody Name: 5D3 [1]

Supplier: Developmental Systems Hybridoma Bank

Comments: IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300

Contributor: Zorn Lab and V Ponferrada

Additional Information: MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work

Reference(s):

applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

@@ Line 1: / Line 1: @@
-=secondary vessel=
+'''Antigen:'''		E-cadherin
-This is the community wiki page for the ''secondary vessel'' please feel free to add any information that is relevant to this anatomy item that is not already captured elsewhere in Xenbase
+'''Epitope:'''		140 kDa xenopus E-cadherin
+'''Host Species/Isotype:'''		mouse monoclonal IgG2b
+'''Antigen Species:'''		xenopus
+'''Applications:'''		IF
+'''Antibody Name:'''		5D3 [http://dshb.biology.uiowa.edu/cadherin-E-]
+'''Supplier:'''		Developmental Systems Hybridoma Bank
+'''Comments:'''		IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300
+'''Contributor:'''		Zorn Lab and V Ponferrada
+'''Additional Information:'''		MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work
+'''Reference(s):'''
+applications abbreviations:
+WB - Western blotting,
+IP - immunoprecipitation,
+IF - immunofluorescence,
+IHC - immunohistochemistry,
+EMSA - electromobility shift assay,
+ChIP - chromatin immunoprecipitation,
+FCM - flow cytometry