4A6 – pronephros

Antigen: dissected pronephroi, target unknown

Epitope:

Host Species/Isotype: mouse monoclonal IgG

Antigen Species:

Applications: IF, IP, WB

Antibody Name: 4A6

Supplier: Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.

Comments: not sentitive to bleaching, does not function post in situ

Contributor: Generated and characterized by Elizabeth Jones [1]. References: Dev Bio 171:531- [2]

applications abbreviations: WB - Western blotting, IP - immunoprecipitation, IF - immunofluorescence, IHC - immunohistochemistry, EMSA - electromobility shift assay, ChIP - chromatin immunoprecipitation, FCM - flow cytometry

Additional Comments:

Developmental Window: First detects target at stage 39, continues until latest stages tested. Stains:' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias. Fixatives: MEMFA Available as:' cell supernatant, 3 x Optimized conditions: Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. Other species: Xenopus only

References: Dev Bio 171:531- [3]