12/101 – somites (monoclonal): Difference between revisions
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(Created page with "Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article....") |
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Revision as of 10:43, 4 February 2011
Generated and characterized by Kintner [1] and Brockes (1985) [2]
Antibody name: 12/101
Raised in: mouse
Subtype: IgG
Antigen: homogenized tissue
Fixatives: MEMFA, Dent's, TCA
Sensitive to bleaching: No
Functions post in situ?: Yes
Immunoprecipitation/Western blotting: 120,000 Mr target
Available as: cell supernatant, 3 x or as ascites
Available from: Developmental Studies Hybridoma Bank [3]
Optimized conditions: Very flexible
Other species: Many- chick, mouse, newt, rabbit
Immunofluorescence using 12/101
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.