Anti-FLAG M2 monoclonal antibody: Difference between revisions

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'''Antigen:''' FLAG epitope
'''Antigen:''' FLAG epitope
'''Xenbase Gene Page:'''
'''Epitope:''' aa DYKDDDDK
'''Epitope:''' aa DYKDDDDK
'''Host Species/Isotype:''' mouse monoclonal, IgG1
'''Host Species/Isotype:''' mouse monoclonal
'''Antigen Species:''' synthetic peptide
'''Antigen Species:''' synthetic peptide
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'''Applications:''' IP, IF, WB
'''Applications:''' IP, IF, WB
'''Antibody Name:''' F3165 [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=F3165|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC]
'''Antibody Name/Catalog Number:''' [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=F3165|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC F3165]
'''Supplier:''' Sigma
'''Supplier:''' Sigma
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'''Reference(s):'''
'''Reference(s):'''

Latest revision as of 15:52, 8 March 2011

Antigen: FLAG epitope

Xenbase Gene Page:

Epitope: aa DYKDDDDK

Host Species/Isotype: mouse monoclonal

Antigen Species: synthetic peptide

Applications: IP, IF, WB

Antibody Name/Catalog Number: F3165

Supplier: Sigma

Comments: IP: use 2 ug, IF: use at 1:1000, WB use at 1:5000 to 1:10,000

Contributor: Zorn Lab and V Ponferrada

Additional Information: IF: MEMFA, 10% TCA fixatives work

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry