4A6 – pronephros (monoclonal): Difference between revisions
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Latest revision as of 10:45, 4 February 2011
Generated and characterized by Elizabeth Jones [1]. References: Dev Bio 171:531- [2]
Antibody name: 4A6
Raised in: mouse, monoclonal
Subtype: IgG
Antigen: dissected pronephroi. Specific target unknown.
Developmental Window: First detects target at stage 39, continues until latest stages tested.
Stains: distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.
Fixatives: MEMFA
Sensitive to bleaching: No
Functions post in situ?: No
Immunoprecipitation/Western blotting: Works with both
Available as: cell supernatent, 3 x
Available from; Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.
Optimized conditions: Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.
Other species: Xenopus only
References: Dev Bio 171:531- [3]