Phospho-histone H3 - mitotic cells: Difference between revisions
Jump to navigation
Jump to search
(Created page with "Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do...") |
(No difference)
|
Revision as of 10:46, 4 February 2011
Generated and characterized by Hendzel et al. [1] First use in Xenopus- Saka and Smith 2001 [2]
Antibody name: Anti-phospho-Histone H3 (Ser10)
Raised in: rabbit
Subtype: IgG
Antigen: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
Fixatives: MEMFA
Sensitive to bleaching: No
Functions post in situ?: Yes
Immunoprecipitation/Western blotting: Yes, very good, 17kD target
Available as: 1 mg/ml solution.
Available from: Upstate, Cat # 06-570 [3]
Optimized conditions: Working dilution approx 1/500
Other species: Pretty much everything from tetrahymena to humans
Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)