Phospho-histone H3 - mitotic cells: Difference between revisions

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Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
'''Antigen:''' phosporylated histone H3 (Ser10)
'''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
'''Host Species/Isotype:'''
'''Antigen Species:''' human
'''Applications:''' IF, WB
'''Antibody Name:'''
'''Supplier:''' Upstate, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570]
'''Comments:''' not sentitive to bleaching, works post in situ
'''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]


applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry


'''Antibody name:''' Anti-phospho-Histone H3 (Ser10)
Additional Comments:
 
''Immunoprecipitation/Western blotting:''' Yes, very good, 17kD target
'''Raised in:''' rabbit
 
'''Subtype:''' IgG
 
'''Antigen:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
 
'''Fixatives:''' MEMFA
 
'''Sensitive to bleaching:''' No
 
'''Functions post in situ?:''' Yes
 
'''Immunoprecipitation/Western blotting:''' Yes, very good, 17kD target
 
'''Available as:''' 1 mg/ml solution.
 
'''Available from:''' Upstate, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570]
 
'''Optimized conditions:''' Working dilution approx 1/500
'''Optimized conditions:''' Working dilution approx 1/500
'''Other species:''' Pretty much everything from tetrahymena to humans
'''Other species:''' Pretty much everything from tetrahymena to humans


[[File:1H3.jpg]] Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)
[[File:1H3.jpg]] Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)

Latest revision as of 13:41, 4 February 2011

Antigen: phosporylated histone H3 (Ser10)

Epitope: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

Host Species/Isotype:

Antigen Species: human

Applications: IF, WB

Antibody Name:

Supplier: Upstate, Cat # 06-570 [1]

Comments: not sentitive to bleaching, works post in situ

Contributor: Generated and characterized by Hendzel et al. [2] First use in Xenopus- Saka and Smith 2001 [3]


applications abbreviations: WB - Western blotting, IP - immunoprecipitation, IF - immunofluorescence, IHC - immunohistochemistry, EMSA - electromobility shift assay, ChIP - chromatin immunoprecipitation, FCM - flow cytometry

Additional Comments: Immunoprecipitation/Western blotting:' Yes, very good, 17kD target Optimized conditions: Working dilution approx 1/500 Other species: Pretty much everything from tetrahymena to humans

Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)

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