Lab Solutions and Reagents: Difference between revisions

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*'''[[microinjection]]'''
*'''[[microinjection]]'''
*'''[[general buffers and salt solutions]]'''
*'''[[general buffers and salt solutions]]'''
 
:HEPES 1 M, pH 7.4
:1M MgCl2
:10x Phosphate Buffered Saline (PBS)
:20x Phosphate Buffered Saline (PBS)
:PBT
:Proteinase K(PK), 10 mg/ml
:PTw
:4M NaCl, 1 liter
:SDS 10%
:TAE 50X
:1M Tris pH 9.5





Revision as of 14:50, 12 December 2011

most information adapted from:

Early Development of Xenopus Laevis: A Laboratory Manual.[1]

2000; (First ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

Sive, Hazel (Author), Grainger, Robert M (Author), and Harland, Richard M (Author).


click each bulleted list heading to see solution/reagent recipes

Modified Barth's Saline (MBS)
Anitbiotics
Gentamycin
Oxytetracycline
Penicillin
Streptomycin Sulfate
High-salt MBS
Amphibian Ringers
Modified Frog Ringers (MR)
Marc's Modified Ringer's (MMR)
Normal Amphibian Medium (NAM)
Holtfreter's Solution
MEMFA
Dent's Fix (Methanol/DMSO)
Bleaching solution (Mayor et al. 1995)
Goat Serum (GS)
PBT
DAB
H2O2
Secondary Antibodies Coupled to Horseradish Peroxidase
Murray's Clearing Medium
Nucleotide Stocks
5x SP6 Buffer
MEMFA
PTw
Triethanolamine 0.1 M
PTw Containing 4% Paraformaldehyde
Hybridization Buffer
SSC, 20X
Denhardt’s solution, 100X
RNase A
RNase T1
4% BMB Blocking Reagent in MAB
Alkaline Phosphatase Buffer
Levamisol 1 M
NBT/BCIP
PVA Alkaline Phosphatase Buffer
Bouin’s Fixative
Bleaching solution (Mayor et al. 1995)
MEMFA
Bouin’s Fixative
Dent's Fix (Methanol/DMSO)
Paraformaldehyde(PFA), 20%
PTw Containing 4% Paraformaldehyde
HEPES 1 M, pH 7.4
1M MgCl2
10x Phosphate Buffered Saline (PBS)
20x Phosphate Buffered Saline (PBS)
PBT
Proteinase K(PK), 10 mg/ml
PTw
4M NaCl, 1 liter
SDS 10%
TAE 50X
1M Tris pH 9.5


agarose plates 1% - for use with laevis or tropicalis
Alkaline Phosphatase (AP) Buffer, with Levamisol and Tween
amphibian Ringers, 5 gal (19 L)
bleaching solution (Sive et al. 2000, Mayor et al. 1995)
4% BMB blocking reagent in MAB
Bouin’s fixative
cysteine, 2.0%
dehydrating embryos
Denhardt’s solution, 100X
dNTPs 10 mM
2.5% Ficoll
2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)
3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)
gentamicin stock, Sigma (1000X, 100 mg/ml)
HEPES 1 M, pH 7.4
hybridization buffer
levamisol 1 M
MAB, 10X
1M MgCl2
MEMFA* (Sive et al. 2000)
100X MR salts
1/3X Modified Ringers
1/9X MR + 1% Gentamicin
1/20X MR
modified Ringers
paraformaldehyde(PFA), 20%
phosphate buffered saline (PBS), 10X
proteinase K(PK), 10 mg/ml
PTw
rehydrating embryos (for embryos stored in 100% methanol)
RNase A (Sigma R 5000)
RNase T1 (Sigma R8251)
4M NaCl, 1 liter
SDS 10%
Sodium Phosphate Buffer(1M)
SSC, 20X
TAE 50X
Triethanolamine 0.1 M (pH7.0-8.0)
1M Tris pH 9.5