Benzocaine stock, 100x (Khokha lab): Difference between revisions

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imported>Virgilio
(Created page with "submitted by VGP from Khokha lab protocols [http://khokha.medicine.yale.edu/protocols_general/Protocol_100XBenzocaine/protocol_100XBenzocaine.html] 100X Benzocaine Sigma Cat ...")
 
imported>Christina
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submitted by VGP
submitted by VGP


from Khokha lab protocols [http://khokha.medicine.yale.edu/protocols_general/Protocol_100XBenzocaine/protocol_100XBenzocaine.html]
Source: Khokha lab protocols [http://khokha.medicine.yale.edu/protocols_general/Protocol_100XBenzocaine/protocol_100XBenzocaine.html]
 


100X Benzocaine
100X Benzocaine
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100X stock is 5% = 5 g/100 ml 100% ethanol
100X stock is 5% = 5 g/100 ml 100% ethanol


1X working solution is 0.05% = 0.05g/100ml 100% ethanol
Dilute to 1X working solution with DI H2O
 
Final solution is 0.05% = 0.05g/100ml 1% ethanol
 
pH should be corrected to 6.8 - 7.0 with NaHCO3.


Store at -20C
Store at -20C
==corrections==
March 8th, 2017: previous version erroneously listed final ethanol conc. as 100% (instead of 1%) and omitted pH correction information.

Latest revision as of 08:48, 8 March 2017

submitted by VGP

Source: Khokha lab protocols [1]

100X Benzocaine

Sigma Cat #E1501-500g (500 g tub)

100X stock is 5% = 5 g/100 ml 100% ethanol

Dilute to 1X working solution with DI H2O

Final solution is 0.05% = 0.05g/100ml 1% ethanol

pH should be corrected to 6.8 - 7.0 with NaHCO3.

Store at -20C

corrections

March 8th, 2017: previous version erroneously listed final ethanol conc. as 100% (instead of 1%) and omitted pH correction information.