XenWiki - User contributions [en]

Centrin 1 - basal bodies

2010-06-22T02:49:50Z

128.83.136.140: Created page with ' '''Antibody name: Centrin 1''' '''Raised in: Rabbit''' '''Subtype:''' '''Antigen: Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centri…'

'''Antibody name: Centrin 1'''

'''Raised in: Rabbit'''

'''Subtype:'''

'''Antigen: Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centrin 1, with N-terminally added lysine. This sequence is highly conserved in centrin 1 of many species, e.g. mouse, drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3'''

'''Fixatives: MEMFA, DENT's'''

'''Sensitive to bleaching:'''

'''Functions post in situ?:'''

'''Immunoprecipitation/Western blotting:'''

'''Available as:'''

'''Available from: Abcam (ab11257)'''

'''Optimized conditions:'''

'''Other species:'''

Gamma-tubulin - Basal bodies, microtubule minus ends

2010-06-22T02:48:11Z

128.83.136.140:

'''Antibody name: Gamma-tubulin (TU-30)'''

'''Raised in: Mouse'''

'''Subtype: IgG'''

'''Antigen: Synthetic peptide: EYHAATRPDYISWGTQ conjugated to KLH, corresponding to amino acids 434/449 of Human gamma Tubulin'''

'''Fixatives: MEMFA, Dent's'''

'''Sensitive to bleaching:'''

'''Functions post in situ?:'''

'''Immunoprecipitation/Western blotting:'''

'''Available as:'''

'''Available from: Abcam (ab27076)'''

'''Optimized conditions:'''

'''Other species:'''

Gamma-tubulin - Basal bodies, microtubule minus ends

2010-06-22T02:47:43Z

128.83.136.140: Created page with 'To use this template, click the "edit" tab, and copy the fields below into the new page you wish to create. '''Antibody name: Gamma-tubulin (TU-30)''' '''Raised in: Mouse''' …'

To use this template, click the "edit" tab, and copy
the fields below into the new page you wish to create.

'''Antibody name: Gamma-tubulin (TU-30)'''

'''Raised in: Mouse'''

'''Subtype: IgG'''

'''Antigen: Synthetic peptide: EYHAATRPDYISWGTQ conjugated to KLH, corresponding to amino acids 434/449 of Human gamma Tubulin'''

'''Fixatives: MEMFA, Dent's'''

'''Sensitive to bleaching:'''

'''Functions post in situ?:'''

'''Immunoprecipitation/Western blotting:'''

'''Available as:'''

'''Available from: Abcam (ab27076)'''

'''Optimized conditions:'''

'''Other species:'''

Antibodies and lectins

2010-06-22T02:43:49Z

128.83.136.140:

'''Antibodies'''

[[Monoclonal 3G8- pronephros and otic vesicle]]

[[Monoclonal 4A6 - pronephros]]

[[Monoclonal 12/101 - somites]]

[[Polyclonal anti-phospho-H3 - mitotic cells]]

[[Alpha-tubulin - For labeling microtubules]]

[[Gamma-tubulin - Basal bodies, microtubule minus ends]]

[[Centrin 1 - basal bodies]]

[[ZO-1]]

[[antibody template]]

'''Lectins'''

[[ECL - stains pronephric nephrons]]

[[LEL - all pronephric epithelia- also faint lateral line]]

[[PNA - stains cement gland and scattered epidermal cells]]

Alpha-tubulin - For labeling microtubules

2010-06-21T16:49:19Z

128.83.136.140:

'''Antibody name:''' DM1A

'''Raised in:''' Mouse

'''Subtype:''' IgG

'''Antigen:''' Microtubules from chick embryo brain

'''Fixatives:''' Works well in MEMFA or Dent's

'''Sensitive to bleaching:''' Yes

'''Functions post in situ?:''' Yes

'''Immunoprecipitation/Western blotting:'''

'''Available as:''' Ascites

'''Available from:''' Sigma (cat#T9026)

'''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.

'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse

Antibodies and lectins

2010-06-21T16:48:50Z

128.83.136.140:

'''Antibodies'''

[[Monoclonal 3G8- pronephros and otic vesicle]]

[[Monoclonal 4A6 - pronephros]]

[[Monoclonal 12/101 - somites]]

[[Polyclonal anti-phospho-H3 - mitotic cells]]

[[Alpha-tubulin - For labeling microtubules]]

[[Gamma-tubulin - Basal bodies microtubule minus ends]]

[[Centrin 1 - basal bodies]]

[[ZO-1]]

[[antibody template]]

'''Lectins'''

[[ECL - stains pronephric nephrons]]

[[LEL - all pronephric epithelia- also faint lateral line]]

[[PNA - stains cement gland and scattered epidermal cells]]

Wholemount antibody staining (CSH Protocols) (Wallingford lab)

2010-06-21T16:46:46Z

128.83.136.140: Created page with 'There are currently several published protocols for this. We (Wallingford lab) have recently published a protocol in CSH Protocols: http://cshprotocols.cshlp.org/cgi/content/fu…'

There are currently several published protocols for this. We (Wallingford lab) have recently published a protocol in CSH Protocols:

http://cshprotocols.cshlp.org/cgi/content/full/2008/3/pdb.prot4957?maxtoshow=&hits=10&RESULTFORMAT=&fulltext=wallingford&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT

That protocol draws from the many excellent previous protcols including:

Robinson, C. and Guille, M. 1999. Immunohistochemistry of Xenopus embryos. Methods Mol. Biol. 127: 89–97.

Sive, H.L., Grainger, R.M., and Harland, R.M. 2000. Early development of Xenopus laevis: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

Becker, B.E and Gard, D.L. 2006. Visualization of the cytoskeleton in Xenopus oocytes and eggs by confocal immunofluorescence microscopy. Methods Mol. Biol. 322: 69–86.

ZO-1

2010-06-21T16:39:01Z

128.83.136.140: Created page with ''''Antibody name: ZO-1''' '''Raised in: Rabbit''' '''Subtype:''' '''Antigen: Amino acids 463-1109 of human ZO-1 cDNA ''' '''Fixatives: MEMFA''' '''Sensitive to bleaching…'

'''Antibody name: ZO-1'''

'''Raised in: Rabbit'''

'''Subtype:'''

'''Antigen: Amino acids 463-1109 of human ZO-1 cDNA '''

'''Fixatives: MEMFA'''

'''Sensitive to bleaching:'''

'''Functions post in situ?:'''

'''Immunoprecipitation/Western blotting:'''

'''Available as:'''

'''Available from: Invitrogen (cat# 61-7300)'''

'''Optimized conditions:'''

'''Other species:'''

Antibodies and lectins

2010-06-21T16:37:11Z

128.83.136.140:

'''Antibodies'''

[[Monoclonal 3G8- pronephros and otic vesicle]]

[[Monoclonal 4A6 - pronephros]]

[[Monoclonal 12/101 - somites]]

[[Polyclonal anti-phospho-H3 - mitotic cells]]

[[Alpha-tubulin - For labeling microtubules]]

[[Gamma-tubulin]]

[[Centrin 1]]

[[ZO-1]]

[[antibody template]]

'''Lectins'''

[[ECL - stains pronephric nephrons]]

[[LEL - all pronephric epithelia- also faint lateral line]]

[[PNA - stains cement gland and scattered epidermal cells]]

Centrin 1

2010-06-21T16:34:52Z

128.83.136.140: Created page with 'To use this template, click the "edit" tab, and copy the fields below into the new page you wish to create. '''Antibody name: Centrin 1 (ab11257)''' '''Raised in: rabbit''' …'

To use this template, click the "edit" tab, and copy
the fields below into the new page you wish to create.

'''Antibody name: Centrin 1 (ab11257)'''

'''Raised in: rabbit'''

'''Subtype: IgG'''

'''Antigen: Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centrin 1, with N-terminally added lysine. This sequence is highly conserved in centrin 1 of many species, e.g. mouse, drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3. '''

'''Fixatives: MEMFA, DENT's'''

'''Sensitive to bleaching:'''

'''Functions post in situ?:'''

'''Immunoprecipitation/Western blotting:'''

'''Available as:'''

'''Available from: ABcam (ab11257)'''

'''Optimized conditions:'''

'''Other species:'''

Antibodies and lectins

2010-06-21T16:32:45Z

128.83.136.140:

'''Antibodies'''

[[Monoclonal 3G8- pronephros and otic vesicle]]

[[Monoclonal 4A6 - pronephros]]

[[Monoclonal 12/101 - somites]]

[[Polyclonal anti-phospho-H3 - mitotic cells]]

[[Alpha-tubulin - For labeling microtubules]]

[[Gamma-tubulin]]

[[Centrin 1]]

[[antibody template]]

'''Lectins'''

[[ECL - stains pronephric nephrons]]

[[LEL - all pronephric epithelia- also faint lateral line]]

[[PNA - stains cement gland and scattered epidermal cells]]

Antibodies and lectins

2010-06-21T16:32:37Z

128.83.136.140:

'''Antibodies'''

[[Monoclonal 3G8- pronephros and otic vesicle]]

[[Monoclonal 4A6 - pronephros]]

[[Monoclonal 12/101 - somites]]

[[Polyclonal anti-phospho-H3 - mitotic cells]]

[[Alpha-tubulin - For labeling microtubules]]

[[Gamma-tubulin]]

[[Cetrin 1]]

[[antibody template]]

'''Lectins'''

[[ECL - stains pronephric nephrons]]

[[LEL - all pronephric epithelia- also faint lateral line]]

[[PNA - stains cement gland and scattered epidermal cells]]

XB-FEAT-491265

2010-06-21T16:32:04Z

128.83.136.140:

To use this template, click the "edit" tab, and copy
the fields below into the new page you wish to create.

'''Antibody name: gamma-tubulin antibody (TU-30)'''

'''Raised in: Mouse'''

'''Subtype:IgG'''

'''Antigen: Synthetic peptide: EYHAATRPDYISWGTQ conjugated to KLH, corresponding to amino acids 434/449 of Human gamma Tubulin'''

'''Fixatives: MEMFA, DENT's'''

'''Sensitive to bleaching:'''

'''Functions post in situ?:'''

'''Immunoprecipitation/Western blotting:'''

'''Available as:'''

'''Available from: Abcam (ab27076)'''

'''Optimized conditions:'''

'''Other species: Hu, Ms, Rat, Chk, Pig, Xl'''

Antibodies and lectins

2010-06-21T16:28:44Z

128.83.136.140:

'''Antibodies'''

[[Monoclonal 3G8- pronephros and otic vesicle]]

[[Monoclonal 4A6 - pronephros]]

[[Monoclonal 12/101 - somites]]

[[Polyclonal anti-phospho-H3 - mitotic cells]]

[[Alpha-tubulin - For labeling microtubules]]

[[Gamma-tubulin]]

[[antibody template]]

'''Lectins'''

[[ECL - stains pronephric nephrons]]

[[LEL - all pronephric epithelia- also faint lateral line]]

[[PNA - stains cement gland and scattered epidermal cells]]

Antibodies and lectins

2010-06-21T16:21:07Z

128.83.136.140:

'''Antibodies'''

[[Monoclonal 3G8- pronephros and otic vesicle]]

[[Monoclonal 4A6 - pronephros]]

[[Monoclonal 12/101 - somites]]

[[Polyclonal anti-phospho-H3 - mitotic cells]]

[[Alpha-tubulin - For labeling microtubules]]

[[antibody template]]

'''Lectins'''

[[ECL - stains pronephric nephrons]]

[[LEL - all pronephric epithelia- also faint lateral line]]

[[PNA - stains cement gland and scattered epidermal cells]]

Antibodies and lectins

2010-06-21T15:34:08Z

128.83.136.140:

'''Antibodies'''

[[Monoclonal 3G8- pronephros and otic vesicle]]

[[Monoclonal 4A6 - pronephros]]

[[Monoclonal 12/101 - somites]]

[[Polyclonal anti-phospho-H3 - mitotic cells]]

[[Alpha-tubulin - For labeling microtubules]]

[[GFP - For post-fixation imaging of GFP-fusions]]

[[antibody template]]

'''Lectins'''

[[ECL - stains pronephric nephrons]]

[[LEL - all pronephric epithelia- also faint lateral line]]

[[PNA - stains cement gland and scattered epidermal cells]]

Antibodies and lectins

2010-06-18T18:36:52Z

128.83.136.140:

'''Antibodies'''

[[Monoclonal 3G8- pronephros and otic vesicle]]

[[Monoclonal 4A6 - pronephros]]

[[Monoclonal 12/101 - somites]]

[[Polyclonal anti-phospho-H3 - mitotic cells]]

[[Alpha-tubulin - For labeling microtubules]]

[[antibody template]]

'''Lectins'''

[[ECL - stains pronephric nephrons]]

[[LEL - all pronephric epithelia- also faint lateral line]]

[[PNA - stains cement gland and scattered epidermal cells]]

13th International Xenopus Conference

2010-06-18T18:22:52Z

128.83.136.140:

Please set aside September 12 -16, 2010 for the 13th International Xenopus Conference. The meeting will be held at the Chateau Lake Louise, a spectacular 5 star Fairmont hotel overlooking the lake and glacier and surrounded by fantastic hikes. September is early Fall and the forests of Banff National Park will be in spendid golden color. While there will be snow on the mountain tops the weather should be very mild. Special rates have been negotiated with the Chateau for rooms and meals for the meeting plus for 3 days prior and following, should you wish to spend some additional vacation time at this amazing venue. Guests are welcome. White water rafting, heli-skiing, climbing and other options are available. A hostel is located 2 km down the road for those on a limited budget.

This year, in addition to the usual line-up of great talks, there will be several informal sessions centered on the Xenopus community. these will include round-table discussions for postdocs approaching the job market and for assistant professors getting started and looking towards tenure. Look for announcements for these discussions at the meeting. In addition, on the 16th, immediately after the conference, we be holding a PIs meeting for Xenopus resource development, where we will begin setting priorities for the next iteration of the Xenopus White Paper (http://www.xenopus2010.org/xenopus2010/xrd.do).

Costs are still being calculated and negotiated- but a working estimate for planning purposes is shared room and all meals US $ 800 / CN $1000 (all costs inc. taxes and gratuities). Private rooms will be available as will a meal only plan for guests. We will offer subsidized rates for graduate students through grant applications that are in progress.

Some free time for hiking will be available each afternoon and more adventurous options available on one afternoon. Evenings will have full lectures by featured speakers followed by poster sessions.

Direct flights to Calgary (the serving airport, code YYC) are available from multiple international destinations incluing Amsterdam, Basel, Frankfurt, London, Machester, Paris and others. In those cases where direct flights are not available, non-stop flights to Calgary from major Canadian and USA hubs are available including Vancouver, Seattle, San Francisco (all good for flights from Asia), Dallas, Chicago, Denver, Toronto and many others. Buses will transport attendees from Calgary to Lake Louise and multiple shuttles and rental car agencies offer flexible alternatives for local travel. More travel details will be posted at a later date.

Preliminary invitations for speakers have been sent out and most slots are full. The organizers are planning on supporting new laboratories and new faculty in selecting speakers and we request that speakers from the 12th Xenopus conference understand that labs that were not represented in Germany have priority this year.

Antibody template

2010-06-16T17:07:09Z

128.83.136.140:

Alpha-tubulin antibody for labeling microtubules

'''Antibody name:''' DM1A

'''Raised in:''' Mouse

'''Subtype:''' IgG

'''Antigen:''' Microtubules from chick embryo brain

'''Fixatives:''' Works well in MEMFA or Dent's

'''Sensitive to bleaching:''' Yes

'''Functions post in situ?:''' Yes

'''Immunoprecipitation/Western blotting:'''

'''Available as:''' Ascites

'''Available from:''' Sigma (cat#T9026)

'''Optimized conditions:''' We dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room tempp for four hours.

'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse