https://wiki.xenbase.org/xenwiki/api.php?action=feedcontributions&user=205.142.197.91&feedformat=atom
XenWiki - User contributions [en]
2024-03-29T08:47:21Z
User contributions
MediaWiki 1.40.1
https://wiki.xenbase.org/xenwiki/index.php?title=Zn-12_-_neuronal_marker&diff=47101
Zn-12 - neuronal marker
2011-02-07T14:51:39Z
<p>205.142.197.91: Created page with "'''Antigen:''' neuronal cell surface marker '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG1 '''Antigen Species:''' zebrafish '''Applicat..."</p>
<hr />
<div>'''Antigen:''' neuronal cell surface marker<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG1<br />
<br />
'''Antigen Species:''' zebrafish<br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' zn-12<br />
<br />
'''Supplier:''' DSHB<br />
<br />
'''Comments:''' works well for IF, fixation: Dent's<br />
<br />
'''Contributor:''' Sandra Kolker and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=637
Antibodies and lectins
2011-02-07T14:50:58Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[zn-12 - neuronal marker]]<br />
<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[collagen type II - col2a1 - ECM]]<br />
<br />
[[collagen type II - col2a1 - ECM broad]]<br />
<br />
[[eya1 - eya1 - developing ear]]<br />
<br />
[[fibrillin-2 - fbn2 - secreted from endothelium]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[hnk-1 - b3gat1 - migrating neural crest]]<br />
<br />
[[slug - snai2 - endothelial to mesenchyme transition, neural crest]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin I - tnni3 - heart]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[acetylated alpha-tubulin - tuba4b]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Eya1_-_eya1_-_developing_ear&diff=47096
Eya1 - eya1 - developing ear
2011-02-07T14:39:49Z
<p>205.142.197.91: Created page with "'''Antigen:''' drosophila eya1 '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG1 '''Antigen Species:''' drosophila '''Applications:''' I..."</p>
<hr />
<div>'''Antigen:''' drosophila eya1<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG1<br />
<br />
'''Antigen Species:''' drosophila<br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' Eya10H6<br />
<br />
'''Supplier:''' DSHB<br />
<br />
'''Comments:''' works for IF but not well, fixative: Dent's<br />
<br />
'''Contributor:''' Sandra Kolker and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=636
Antibodies and lectins
2011-02-07T14:39:23Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[collagen type II - col2a1 - ECM]]<br />
<br />
[[collagen type II - col2a1 - ECM broad]]<br />
<br />
[[eya1 - eya1 - developing ear]]<br />
<br />
[[fibrillin-2 - fbn2 - secreted from endothelium]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[hnk-1 - b3gat1 - migrating neural crest]]<br />
<br />
[[slug - snai2 - endothelial to mesenchyme transition, neural crest]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin I - tnni3 - heart]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[acetylated alpha-tubulin - tuba4b]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Acetylated_alpha-tubulin_-_tuba4b&diff=47094
Acetylated alpha-tubulin - tuba4b
2011-02-07T14:33:48Z
<p>205.142.197.91: Created page with "'''Antigen:''' acetylated tubulin '''Epitope:''' acetylated alpha-tubulin from the outer arm of sea urchin axonemes '''Host Species/Isotype:''' mouse monoclonal IgG..."</p>
<hr />
<div>'''Antigen:''' acetylated tubulin<br />
<br />
'''Epitope:''' acetylated alpha-tubulin from the outer arm of sea urchin axonemes<br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG2B<br />
<br />
'''Antigen Species:''' sea urchin<br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' 6-11B-1<br />
<br />
'''Supplier:''' Sigma<br />
<br />
'''Comments:''' works well for IF, fixation: Dent's or PFA<br />
<br />
'''Contributor:''' <br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=635
Antibodies and lectins
2011-02-07T14:33:25Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[collagen type II - col2a1 - ECM]]<br />
<br />
[[collagen type II - col2a1 - ECM broad]]<br />
<br />
[[fibrillin-2 - fbn2 - secreted from endothelium]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[hnk-1 - b3gat1 - migrating neural crest]]<br />
<br />
[[slug - snai2 - endothelial to mesenchyme transition, neural crest]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin I - tnni3 - heart]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[acetylated alpha-tubulin - tuba4b]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Hnk-1_-_b3gat1_-_migrating_neural_crest&diff=47091
Hnk-1 - b3gat1 - migrating neural crest
2011-02-07T14:27:39Z
<p>205.142.197.91: Created page with "'''Antigen:''' human natural killer cell glycoprotein '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgM '''Antigen Species:''' human '''App..."</p>
<hr />
<div>'''Antigen:''' human natural killer cell glycoprotein<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgM<br />
<br />
'''Antigen Species:''' human<br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' MS-1163<br />
<br />
'''Supplier:''' Neomarkers<br />
<br />
'''Comments:''' works well for IF, fixative: Dent's or PFA<br />
<br />
'''Contributor:''' Sandra Kolker and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments: marker for migrating nerual crest cells and cells of the 2 field moving into myocardium</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=634
Antibodies and lectins
2011-02-07T14:26:49Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[collagen type II - col2a1 - ECM]]<br />
<br />
[[collagen type II - col2a1 - ECM broad]]<br />
<br />
[[fibrillin-2 - fbn2 - secreted from endothelium]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[hnk-1 - b3gat1 - migrating neural crest]]<br />
<br />
[[slug - snai2 - endothelial to mesenchyme transition, neural crest]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin I - tnni3 - heart]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Slug_-_snai2_-_endothelial_to_mesenchyme_transition,_neural_crest&diff=47088
Slug - snai2 - endothelial to mesenchyme transition, neural crest
2011-02-07T14:20:17Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' chicken slug<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG1 kappa light chain<br />
<br />
'''Antigen Species:''' chicken<br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' 62.1E6<br />
<br />
'''Supplier:''' DSHB<br />
<br />
'''Comments:''' works moderately well for IF, fixative: Dent's<br />
<br />
'''Contributor:''' Sandra Kolker and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Slug_-_snai2_-_endothelial_to_mesenchyme_transition,_neural_crest&diff=47087
Slug - snai2 - endothelial to mesenchyme transition, neural crest
2011-02-07T14:19:17Z
<p>205.142.197.91: Created page with "'''Antigen:''' chicken slug '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG1 kappa light chain '''Antigen Species:''' chicken '''Applicat..."</p>
<hr />
<div>'''Antigen:''' chicken slug<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG1 kappa light chain<br />
<br />
'''Antigen Species:''' chicken<br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' 6.21E+07<br />
<br />
'''Supplier:''' DSHB<br />
<br />
'''Comments:''' works moderately well for IF, fixative: Dent's<br />
<br />
'''Contributor:''' Sandra Kolker and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=633
Antibodies and lectins
2011-02-07T14:18:51Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[collagen type II - col2a1 - ECM]]<br />
<br />
[[collagen type II - col2a1 - ECM broad]]<br />
<br />
[[fibrillin-2 - fbn2 - secreted from endothelium]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[slug - snai2 - endothelial to mesenchyme transition, neural crest]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin I - tnni3 - heart]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Collagen_type_II_-_col2a1_-_ECM_broad&diff=47084
Collagen type II - col2a1 - ECM broad
2011-02-07T14:13:52Z
<p>205.142.197.91: Created page with "'''Antigen:''' chicken type II collagen '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG2a '''Antigen Species:''' chicken '''Applications:..."</p>
<hr />
<div>'''Antigen:''' chicken type II collagen<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG2a<br />
<br />
'''Antigen Species:''' chicken<br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' CIICI<br />
<br />
'''Supplier:''' DSHB<br />
<br />
'''Comments:''' works for IF but not well, fixative: Dent's<br />
<br />
'''Contributor:''' Sandra Kolker and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=632
Antibodies and lectins
2011-02-07T14:13:15Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[collagen type II - col2a1 - ECM]]<br />
<br />
[[collagen type II - col2a1 - ECM broad]]<br />
<br />
[[fibrillin-2 - fbn2 - secreted from endothelium]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin I - tnni3 - heart]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=631
Antibodies and lectins
2011-02-07T14:12:06Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[collagen type II - col2a1 - ECM]]<br />
<br />
[[collagen type II - col2a1 - ECM]]<br />
<br />
[[fibrillin-2 - fbn2 - secreted from endothelium]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin I - tnni3 - heart]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Collagen_type_II_-_col2a1_-_ECM&diff=47081
Collagen type II - col2a1 - ECM
2011-02-07T14:08:48Z
<p>205.142.197.91: Created page with "'''Antigen:''' chicken type II collagen '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG kappa light chain '''Antigen Species:''' chicken ..."</p>
<hr />
<div>'''Antigen:''' chicken type II collagen<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG kappa light chain<br />
<br />
'''Antigen Species:''' chicken<br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' II-II6B3<br />
<br />
'''Supplier:''' DSHB<br />
<br />
'''Comments:''' works moderately well for IF, fixative: Dent's<br />
<br />
'''Contributor:''' Sandra Kolker and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=630
Antibodies and lectins
2011-02-07T14:08:03Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[collagen type II - col2a1 - ECM]]<br />
<br />
[[fibrillin-2 - fbn2 - secreted from endothelium]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin I - tnni3 - heart]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Fibrillin-2_-_fbn2_-_secreted_from_endothelium&diff=47078
Fibrillin-2 - fbn2 - secreted from endothelium
2011-02-07T14:03:52Z
<p>205.142.197.91: Created page with "'''Antigen:''' chicken fibrillin-2 '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG1 '''Antigen Species:''' chicken '''Applications:''' ..."</p>
<hr />
<div>'''Antigen:''' chicken fibrillin-2<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG1<br />
<br />
'''Antigen Species:''' chicken<br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' JB-3<br />
<br />
'''Supplier:''' DSHB<br />
<br />
'''Comments:''' works well for IF, fixative: Dent's or PFA<br />
<br />
'''Contributor:''' Sandra Kolker and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=629
Antibodies and lectins
2011-02-07T14:03:13Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[fibrillin-2 - fbn2 - secreted from endothelium]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin I - tnni3 - heart]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Troponin_-_cardiac_troponin_I_-_tnni3_-_heart&diff=47075
Troponin - cardiac troponin I - tnni3 - heart
2011-02-07T13:57:37Z
<p>205.142.197.91: Created page with " '''Antigen:''' human troponin I '''Epitope:''' '''Host Species/Isotype:''' goat polyclonal '''Antigen Species:''' '''Applications:''' IF ..."</p>
<hr />
<div> '''Antigen:''' human troponin I<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' goat polyclonal<br />
<br />
'''Antigen Species:''' <br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' 70-XG79<br />
<br />
'''Supplier:''' Fitzgerald<br />
<br />
'''Comments:''' works well for IF, fixative: Dent's or PFA<br />
<br />
'''Contributor:''' Sandra Kolker and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=628
Antibodies and lectins
2011-02-07T13:56:53Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin I - tnni3 - heart]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Centrin_1_-_cetn1_-_basal_bodies&diff=47072
Centrin 1 - cetn1 - basal bodies
2011-02-07T13:47:56Z
<p>205.142.197.91: Created page with "'''Antigen:''' centrin 1 '''Epitope:''' Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centrin 1, with N-terminally added lysine. This..."</p>
<hr />
<div>'''Antigen:''' centrin 1<br />
<br />
'''Epitope:''' Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centrin 1, with N-terminally added lysine. This sequence is highly conserved in centrin 1 of many species, e.g. mouse, drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3<br />
<br />
'''Host Species/Isotype:''' rabbit<br />
<br />
'''Antigen Species:''' human<br />
<br />
'''Applications:''' <br />
<br />
'''Antibody Name:''' ab11257<br />
<br />
'''Supplier:''' Abcam<br />
<br />
'''Comments:'''<br />
<br />
'''Contributor:'''<br />
<br />
<br />
applications abbreviations:<br />
WB - Western blotting,<br />
IP - immunoprecipitation,<br />
IF - immunofluorescence,<br />
IHC - immunohistochemistry,<br />
EMSA - electromobility shift assay,<br />
ChIP - chromatin immunoprecipitation,<br />
FCM - flow cytometry</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=627
Antibodies and lectins
2011-02-07T13:47:18Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - cetn1 - basal bodies]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=ZO-1_-_tjp1&diff=47069
ZO-1 - tjp1
2011-02-04T20:17:56Z
<p>205.142.197.91: Created page with "'''Antigen:''' human ZO-1 '''Epitope:''' amino acids 463-1109 of human ZO-1 '''Host Species/Isotype:''' rabbit '''Antigen Species:''' human '''Application..."</p>
<hr />
<div>'''Antigen:''' human ZO-1<br />
<br />
'''Epitope:''' amino acids 463-1109 of human ZO-1<br />
<br />
'''Host Species/Isotype:''' rabbit<br />
<br />
'''Antigen Species:''' human<br />
<br />
'''Applications:''' WB, IHC, ELISA, IHC<br />
<br />
'''Antibody Name:''' ZO-1<br />
<br />
'''Supplier:''' Invitrogen (cat# 61-7300)<br />
<br />
'''Comments:''' reactive in human, mouse, rat, chicken, guinea pig<br />
<br />
'''Contributor:''' <br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=626
Antibodies and lectins
2011-02-04T20:17:33Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - basal bodies]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1 - tjp1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Gamma-tubulin_-_tubg1_-_basal_bodies,_microtubule_minus_ends&diff=47066
Gamma-tubulin - tubg1 - basal bodies, microtubule minus ends
2011-02-04T20:16:00Z
<p>205.142.197.91: Created page with "'''Antigen:''' human gamma-tubulin '''Epitope:''' Synthetic peptide: EYHAATRPDYISWGTQ conjugated to KLH, corresponding to amino acids 434/449 of Human gamma Tubulin '..."</p>
<hr />
<div>'''Antigen:''' human gamma-tubulin<br />
<br />
'''Epitope:''' Synthetic peptide: EYHAATRPDYISWGTQ conjugated to KLH, corresponding to amino acids 434/449 of Human gamma Tubulin<br />
<br />
'''Host Species/Isotype:''' mouse<br />
<br />
'''Antigen Species:''' human<br />
<br />
'''Applications:''' <br />
<br />
'''Antibody Name:''' TU-30<br />
<br />
'''Supplier:''' Abcam (ab27076)<br />
<br />
'''Comments:''' fixatives: MEMFA, Dent's<br />
<br />
'''Contributor:''' <br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=625
Antibodies and lectins
2011-02-04T20:15:36Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - basal bodies]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Alpha-tubulin_-_tuba4b_-_microtubules&diff=47062
Alpha-tubulin - tuba4b - microtubules
2011-02-04T20:13:30Z
<p>205.142.197.91: Created page with "'''Antigen:''' microtubules from chick embryo brain '''Epitope:''' '''Host Species/Isotype:''' mouse IgG '''Antigen Species:''' chicken '''Applications:'..."</p>
<hr />
<div>'''Antigen:''' microtubules from chick embryo brain<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse IgG<br />
<br />
'''Antigen Species:''' chicken<br />
<br />
'''Applications:''' <br />
<br />
'''Antibody Name:''' DM1A<br />
<br />
'''Supplier:''' Sigma (cat#T9026)<br />
<br />
'''Comments:''' fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ<br />
<br />
'''Contributor:''' Wallingford Lab<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments: <br />
<br />
'''Available as:''' Ascites<br />
<br />
'''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.<br />
<br />
'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=624
Antibodies and lectins
2011-02-04T20:13:07Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - basal bodies]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - tuba4b - microtubules]]<br />
<br />
[[gamma-tubulin - Basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=ZO-1&diff=44505
ZO-1
2011-02-04T20:10:08Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' human ZO-1<br />
<br />
'''Epitope:''' amino acids 463-1109 of human ZO-1<br />
<br />
'''Host Species/Isotype:''' rabbit<br />
<br />
'''Antigen Species:''' human<br />
<br />
'''Applications:''' WB, IHC, ELISA, IHC<br />
<br />
'''Antibody Name:''' ZO-1<br />
<br />
'''Supplier:''' Invitrogen (cat# 61-7300)<br />
<br />
'''Comments:''' reactive in human, mouse, rat, chicken, guinea pig<br />
<br />
'''Contributor:''' <br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Gamma-tubulin_-_Basal_bodies,_microtubule_minus_ends&diff=44512
Gamma-tubulin - Basal bodies, microtubule minus ends
2011-02-04T20:06:25Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' human gamma-tubulin<br />
<br />
'''Epitope:''' Synthetic peptide: EYHAATRPDYISWGTQ conjugated to KLH, corresponding to amino acids 434/449 of Human gamma Tubulin<br />
<br />
'''Host Species/Isotype:''' mouse<br />
<br />
'''Antigen Species:''' human<br />
<br />
'''Applications:''' <br />
<br />
'''Antibody Name:''' TU-30<br />
<br />
'''Supplier:''' Abcam (ab27076)<br />
<br />
'''Comments:''' fixatives: MEMFA, Dent's<br />
<br />
'''Contributor:''' <br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments:</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Alpha-tubulin_-_microtubules&diff=47027
Alpha-tubulin - microtubules
2011-02-04T20:01:19Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' microtubules from chick embryo brain<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse IgG<br />
<br />
'''Antigen Species:''' chicken<br />
<br />
'''Applications:''' <br />
<br />
'''Antibody Name:''' DM1A<br />
<br />
'''Supplier:''' Sigma (cat#T9026)<br />
<br />
'''Comments:''' fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ<br />
<br />
'''Contributor:''' Wallingford Lab<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments: <br />
<br />
'''Available as:''' Ascites<br />
<br />
'''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.<br />
<br />
'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=4A6_%E2%80%93_pronephros&diff=47056
4A6 – pronephros
2011-02-04T19:52:30Z
<p>205.142.197.91: Created page with "'''Antigen:''' dissected pronephroi, target unknown '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG '''Antigen Species:''' '''Applicatio..."</p>
<hr />
<div>'''Antigen:''' dissected pronephroi, target unknown<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG<br />
<br />
'''Antigen Species:''' <br />
<br />
'''Applications:''' IF, IP, WB<br />
<br />
'''Antibody Name:''' 4A6<br />
<br />
'''Supplier:''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.<br />
<br />
'''Comments:''' not sentitive to bleaching, does not function post in situ<br />
<br />
'''Contributor:''' Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments: <br />
<br />
'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested.<br />
''Stains:''' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.<br />
'''Fixatives:''' MEMFA<br />
''Available as:''' cell supernatant, 3 x<br />
'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.<br />
'''Other species:''' Xenopus only<br />
<br />
References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=623
Antibodies and lectins
2011-02-04T19:51:32Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros]]<br />
<br />
[[centrin 1 - basal bodies]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - microtubules]]<br />
<br />
[[gamma-tubulin - Basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Centrin_1_-_basal_bodies&diff=44516
Centrin 1 - basal bodies
2011-02-04T19:43:20Z
<p>205.142.197.91: Created page with "'''Antigen:''' centrin 1 '''Epitope:''' Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centrin 1, with N-terminally added lysine. This..."</p>
<hr />
<div>'''Antigen:''' centrin 1<br />
<br />
'''Epitope:''' Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centrin 1, with N-terminally added lysine. This sequence is highly conserved in centrin 1 of many species, e.g. mouse, drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3<br />
<br />
'''Host Species/Isotype:''' rabbit<br />
<br />
'''Antigen Species:''' human<br />
<br />
'''Applications:''' <br />
<br />
'''Antibody Name:''' ab11257<br />
<br />
'''Supplier:''' Abcam<br />
<br />
'''Comments:'''<br />
<br />
'''Contributor:'''<br />
<br />
<br />
applications abbreviations:<br />
WB - Western blotting,<br />
IP - immunoprecipitation,<br />
IF - immunofluorescence,<br />
IHC - immunohistochemistry,<br />
EMSA - electromobility shift assay,<br />
ChIP - chromatin immunoprecipitation,<br />
FCM - flow cytometry</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=622
Antibodies and lectins
2011-02-04T19:42:47Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros (monoclonal)]]<br />
<br />
[[centrin 1 - basal bodies]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - microtubules]]<br />
<br />
[[gamma-tubulin - Basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Phospho-histone_H3_-_mitotic_cells&diff=47025
Phospho-histone H3 - mitotic cells
2011-02-04T19:41:23Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' phosporylated histone H3 (Ser10)<br />
<br />
'''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3<br />
<br />
'''Host Species/Isotype:''' <br />
<br />
'''Antigen Species:''' human<br />
<br />
'''Applications:''' IF, WB<br />
<br />
'''Antibody Name:''' <br />
<br />
'''Supplier:''' Upstate, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570]<br />
<br />
'''Comments:''' not sentitive to bleaching, works post in situ<br />
<br />
'''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments: <br />
''Immunoprecipitation/Western blotting:''' Yes, very good, 17kD target <br />
'''Optimized conditions:''' Working dilution approx 1/500<br />
'''Other species:''' Pretty much everything from tetrahymena to humans<br />
<br />
[[File:1H3.jpg]] Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Centrin_1_-_basal_bodies&diff=44515
Centrin 1 - basal bodies
2011-02-04T19:33:39Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' centrin 1<br />
<br />
'''Epitope:''' Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centrin 1, with N-terminally added lysine. This sequence is highly conserved in centrin 1 of many species, e.g. mouse, drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3<br />
<br />
'''Host Species/Isotype:''' rabbit<br />
<br />
'''Antigen Species:''' human<br />
<br />
'''Applications:''' <br />
<br />
'''Antibody Name:''' ab11257<br />
<br />
'''Supplier:''' Abcam<br />
<br />
'''Comments:'''<br />
<br />
'''Contributor:'''<br />
<br />
<br />
applications abbreviations:<br />
WB - Western blotting,<br />
IP - immunoprecipitation,<br />
IF - immunofluorescence,<br />
IHC - immunohistochemistry,<br />
EMSA - electromobility shift assay,<br />
ChIP - chromatin immunoprecipitation,<br />
FCM - flow cytometry</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=3G8-_pronephros_and_otic_vesicle&diff=47048
3G8- pronephros and otic vesicle
2011-02-04T19:28:45Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' dissected pro nephroi, specific target unknown<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG<br />
<br />
'''Antigen Species:''' <br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' 3G8<br />
<br />
'''Supplier:''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.<br />
<br />
'''Comments:''' IP unknown, does not work on Westerns<br />
<br />
'''Contributor:''' Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
<br />
Additional Comments: <br />
<br />
'''Developmental Window:''' First detects target at stage 31, continues until latest stages tested. As this antibody stained only the lumen of the pronephric proximal tubules the shape of the organ looks odd in early stages and only begins to look like a pronephros at around stage 34 when a well luminized epithelial tubule network has formed. <br />
<br />
'''Stains:''' proximal pronephric nephron, nephrostomes and otic vesicle, <br />
<br />
'''Fixatives:''' formalin, MEMFA, TCA are all OK. Following fixation sample must be dehydrated with methanol for the antibody to work optimally. Others not tested. The target degrades over time in alcohol, so samples should be stored for less than one month post-fixation.<br />
<br />
'''Sensitive to bleaching:''' No, though store time in methanol may be shortened by bleaching<br />
<br />
'''Functions post in situ?:''' No<br />
<br />
'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. In cleared embryos both pronephroi are often in view, one in focus and one blurred, and this can make photography difficult see for example stage 32 sample above.<br />
<br />
'''Other species:''' works on other anurans (Bombina), and also on axolotls when formalin plus 10% DMSO is used as the fixative, but it isn't nearly as clean as with Xenopus or Bombina. This is probably just a protocol problem. Reported to work on zebrafish.<br />
<br />
'''XAO:''' <br />
<br />
References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=3G8-_pronephros_and_otic_vesicle&diff=47047
3G8- pronephros and otic vesicle
2011-02-04T19:27:04Z
<p>205.142.197.91: Created page with "'''Antigen:''' dissected pro nephroi, specific target unknown '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG '''Antigen Species:''' '''..."</p>
<hr />
<div>'''Antigen:''' dissected pro nephroi, specific target unknown<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG<br />
<br />
'''Antigen Species:''' <br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' 3G8<br />
<br />
'''Supplier:''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.<br />
<br />
'''Comments:''' IP unknown, does not work on Westerns<br />
<br />
'''Contributor:''' Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments: <br />
'''Developmental Window:''' First detects target at stage 31, continues until latest stages tested. As this antibody stained only the lumen of the pronephric proximal tubules the shape of the organ looks odd in early stages and only begins to look like a pronephros at around stage 34 when a well luminized epithelial tubule network has formed. <br />
<br />
'''Stains:''' proximal pronephric nephron, nephrostomes and otic vesicle, <br />
<br />
'''Fixatives:''' formalin, MEMFA, TCA are all OK. Following fixation sample must be dehydrated with methanol for the antibody to work optimally. Others not tested. The target degrades over time in alcohol, so samples should be stored for less than one month post-fixation.<br />
<br />
'''Sensitive to bleaching:''' No, though store time in methanol may be shortened by bleaching<br />
<br />
'''Functions post in situ?:''' No<br />
<br />
'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. In cleared embryos both pronephroi are often in view, one in focus and one blurred, and this can make photography difficult see for example stage 32 sample above.<br />
<br />
'''Other species:''' works on other anurans (Bombina), and also on axolotls when formalin plus 10% DMSO is used as the fixative, but it isn't nearly as clean as with Xenopus or Bombina. This is probably just a protocol problem. Reported to work on zebrafish.<br />
<br />
'''XAO:''' <br />
<br />
References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=621
Antibodies and lectins
2011-02-04T19:26:37Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle]]<br />
<br />
[[4A6 – pronephros (monoclonal)]]<br />
<br />
[[Centrin 1 - basal bodies]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - microtubules]]<br />
<br />
[[gamma-tubulin - Basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=3G8-_pronephros_and_otic_vesicle_(monoclonal)&diff=47022
3G8- pronephros and otic vesicle (monoclonal)
2011-02-04T19:25:59Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' dissected pro nephroi, specific target unknown<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG<br />
<br />
'''Antigen Species:''' <br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' 3G8<br />
<br />
'''Supplier:''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.<br />
<br />
'''Comments:''' IP unknown, does not work on Westerns<br />
<br />
'''Contributor:''' Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
Additional Comments: <br />
'''Developmental Window:''' First detects target at stage 31, continues until latest stages tested. As this antibody stained only the lumen of the pronephric proximal tubules the shape of the organ looks odd in early stages and only begins to look like a pronephros at around stage 34 when a well luminized epithelial tubule network has formed. <br />
<br />
'''Stains:''' proximal pronephric nephron, nephrostomes and otic vesicle, <br />
<br />
'''Fixatives:''' formalin, MEMFA, TCA are all OK. Following fixation sample must be dehydrated with methanol for the antibody to work optimally. Others not tested. The target degrades over time in alcohol, so samples should be stored for less than one month post-fixation.<br />
<br />
'''Sensitive to bleaching:''' No, though store time in methanol may be shortened by bleaching<br />
<br />
'''Functions post in situ?:''' No<br />
<br />
'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. In cleared embryos both pronephroi are often in view, one in focus and one blurred, and this can make photography difficult see for example stage 32 sample above.<br />
<br />
'''Other species:''' works on other anurans (Bombina), and also on axolotls when formalin plus 10% DMSO is used as the fixative, but it isn't nearly as clean as with Xenopus or Bombina. This is probably just a protocol problem. Reported to work on zebrafish.<br />
<br />
'''XAO:''' <br />
<br />
References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=12/101_-_somites,_skeletal_muscle_marker&diff=47044
12/101 - somites, skeletal muscle marker
2011-02-04T19:16:17Z
<p>205.142.197.91: Created page with "'''Antigen:''' homogenized tissue '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG '''Antigen Species:''' newt '''Applications:''' IF, W..."</p>
<hr />
<div>'''Antigen:''' homogenized tissue<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG<br />
<br />
'''Antigen Species:''' newt<br />
<br />
'''Applications:''' IF, WB<br />
<br />
'''Antibody Name:''' 12/101<br />
<br />
'''Supplier:''' Developmental Studies Hybridoma Bank<br />
<br />
'''Comments:''' WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)<br />
<br />
'''Contributor:''' Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article.do?method=display&articleId=40901]<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
[[File:12101.jpg]] <br />
Immunofluorescence using 12/101 <br />
<br />
[[File:12101+ATPase.jpg]]<br />
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=620
Antibodies and lectins
2011-02-04T19:15:44Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 - somites, skeletal muscle marker]]<br />
<br />
[[3G8- pronephros and otic vesicle (monoclonal)]]<br />
<br />
[[4A6 – pronephros (monoclonal)]]<br />
<br />
[[Centrin 1 - basal bodies]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - microtubules]]<br />
<br />
[[gamma-tubulin - Basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=12/101_%E2%80%93_somites_(monoclonal)&diff=47020
12/101 – somites (monoclonal)
2011-02-04T19:14:33Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' homogenized tissue<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG<br />
<br />
'''Antigen Species:''' newt<br />
<br />
'''Applications:''' IF, WB<br />
<br />
'''Antibody Name:''' 12/101<br />
<br />
'''Supplier:''' Developmental Studies Hybridoma Bank<br />
<br />
'''Comments:''' WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)<br />
<br />
'''Contributor:''' Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article.do?method=display&articleId=40901]<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry <br />
<br />
[[File:12101.jpg]] <br />
Immunofluorescence using 12/101 <br />
<br />
[[File:12101+ATPase.jpg]]<br />
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibody_template&diff=693
Antibody template
2011-02-04T19:03:02Z
<p>205.142.197.91: </p>
<hr />
<div>To use this template, click the "edit" tab, and copy <br />
the fields below into the new page you wish to create.<br />
<br />
<br />
'''Antigen:'''<br />
<br />
'''Epitope:'''<br />
<br />
'''Host Species/Isotype:'''<br />
<br />
'''Antigen Species:'''<br />
<br />
'''Applications:'''<br />
<br />
'''Antibody Name:'''<br />
<br />
'''Supplier:'''<br />
<br />
'''Comments:'''<br />
<br />
'''Contributor:'''<br />
<br />
<br />
applications abbreviations:<br />
WB - Western blotting,<br />
IP - immunoprecipitation,<br />
IF - immunofluorescence,<br />
IHC - immunohistochemistry,<br />
EMSA - electromobility shift assay,<br />
ChIP - chromatin immunoprecipitation,<br />
FCM - flow cytometry</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Troponin_-_cardiac_troponin_T_-_tnnt2_-_heart&diff=47041
Troponin - cardiac troponin T - tnnt2 - heart
2011-02-04T18:56:54Z
<p>205.142.197.91: Created page with "'''Antigen:''' cardiac troponin T '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG2a '''Antigen Species:''' bovine '''Applications:''' I..."</p>
<hr />
<div>'''Antigen:''' cardiac troponin T<br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG2a<br />
<br />
'''Antigen Species:''' bovine<br />
<br />
'''Applications:''' IF<br />
<br />
'''Antibody Name:''' CT3<br />
<br />
'''Supplier:''' DSHB<br />
<br />
'''Comments:''' works well for IF, fixative Dent's or PFA<br />
<br />
'''Contributor:''' Sandra Kolker and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Antibodies_and_lectins&diff=619
Antibodies and lectins
2011-02-04T18:55:07Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antibodies'''<br />
<br />
[[Antibody template]]<br />
<br />
[[12/101 – somites (monoclonal)]]<br />
<br />
[[3G8- pronephros and otic vesicle (monoclonal)]]<br />
<br />
[[4A6 – pronephros (monoclonal)]]<br />
<br />
[[Centrin 1 - basal bodies]]<br />
<br />
[[phospho-histone H3 - mitotic cells]]<br />
<br />
[[troponin - heart and other muscle]]<br />
<br />
[[troponin - cardiac troponin T - tnnt2 - heart]]<br />
<br />
[[alpha-tubulin - microtubules]]<br />
<br />
[[gamma-tubulin - Basal bodies, microtubule minus ends]]<br />
<br />
[[ZO-1]]<br />
<br />
<br />
<br />
'''Lectins'''<br />
<br />
[[ECL - pronephric nephrons]]<br />
<br />
[[LEL - all pronephric epithelia- also faint lateral line]]<br />
<br />
[[PNA - cement gland and scattered epidermal cells]]</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Troponin_-_heart_and_other_muscle&diff=47038
Troponin - heart and other muscle
2011-02-04T18:22:45Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' tropomyosin, muscle <br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG1 <br />
<br />
'''Antigen Species:''' chicken <br />
<br />
'''Applications:''' IF <br />
<br />
'''Antibody Name:''' CH1 <br />
<br />
'''Supplier:''' DSHB <br />
<br />
'''Comments:''' works well for IF, fixative Dent's or PFA<br />
<br />
'''Contributor:''' Sandra Koller and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Troponin_-_heart_and_other_muscle&diff=47037
Troponin - heart and other muscle
2011-02-04T18:17:25Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' tropomyosin, muscle <br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG1 <br />
<br />
'''Antigen Species:''' chicken <br />
<br />
'''Applications:''' IF <br />
<br />
'''Antibody Name:''' CH1 <br />
<br />
'''Supplier:''' DSHB <br />
<br />
'''Comments:''' works well in IF, fixative Dent's or PFA<br />
<br />
'''Contributor:''' Sandra Koller and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry</div>
205.142.197.91
https://wiki.xenbase.org/xenwiki/index.php?title=Troponin_-_heart_and_other_muscle&diff=47036
Troponin - heart and other muscle
2011-02-04T18:16:51Z
<p>205.142.197.91: </p>
<hr />
<div>'''Antigen:''' tropomyosin, muscle <br />
<br />
'''Epitope:''' <br />
<br />
'''Host Species/Isotype:''' mouse monoclonal IgG1 <br />
<br />
'''Antigen Species:''' chicken <br />
<br />
'''Applications:''' IF <br />
<br />
'''Antibody Name:''' CH1 <br />
<br />
'''Supplier:''' DSHB <br />
<br />
'''Comments:''' works well in IF, fixative Dent's or PFA<br />
<br />
'''Contributor:''' Sandra Koller and Dan Weeks<br />
<br />
<br />
applications abbreviations: <br />
WB - Western blotting, <br />
IP - immunoprecipitation, <br />
IF - immunofluorescence, <br />
IHC - immunohistochemistry, <br />
EMSA - electromobility shift assay, <br />
ChIP - chromatin immunoprecipitation, <br />
FCM - flow cytometry,</div>
205.142.197.91