View source for 4A6 – pronephros (monoclonal)

Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]


'''Antibody name:''' 4A6

'''Raised in:''' mouse, monoclonal

'''Subtype:''' IgG

'''Antigen:''' dissected pronephroi. Specific target unknown.

'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested.

'''Stains:''' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.

'''Fixatives:''' MEMFA

'''Sensitive to bleaching:''' No

'''Functions post in situ?:''' No

'''Immunoprecipitation/Western blotting:''' Works with both

'''Available as:''' cell supernatent, 3 x

'''Available from;''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.

'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense.  As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.

'''Other species:''' Xenopus only

References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]