View source for Chromosomal FISH-TSA

'''Literature:'''

Krylov V., Macha J., Tlapakova T., Takac M., Jonak J. (2003) The c-SRC1 gene visualized by in situ hybridization on Xenopus laevis chromosomes. Cytogenet. Genome Res. 2003;103(1-2):169-172. [http://www.ncbi.nlm.nih.gov/pubmed/15004482].

Tlapakova T., Krylov V., Macha J.  (2005) Localization, structure and polymorphism of two paralogous Xenopus laevis mitochondrial malate dehydrogenase genes. Chromosome res. 13(7):699-706. [http://www.ncbi.nlm.nih.gov/pubmed/16235119].

Krylov V., Tlapakova T., Macha J.  (2007) Localization of Mdh2 single copy gene on Xenopus tropicalis chromosomes by FISH-TSA technique. Cytogenet. Genome Res. 116:110-112. [http://www.ncbi.nlm.nih.gov/pubmed/17268187].

Following protocol is designed for the visualization of short cDNA sequences with the minimal lenght of 900-1000 bp.

'''Protocol:'''

* [[Image:Krylov_FISH-TSA_protocol.pdf |Data mapping document]]