View source for Phospho-histone H3 - mitotic cells

'''Antigen:'''			phosporylated histone H3 (Ser10)
			
'''Epitope:'''			KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
			
'''Host Species/Isotype:'''			
			
'''Antigen Species:'''			human
			
'''Applications:'''			IF, WB
			
'''Antibody Name:'''			
			
'''Supplier:'''			Upstate, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570]
			
'''Comments:'''			not sentitive to bleaching, works post in situ
			
'''Contributor:'''			Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
			

applications abbreviations:			
WB - Western blotting,			
IP - immunoprecipitation,			
IF - immunofluorescence,			
IHC - immunohistochemistry,			
EMSA - electromobility shift assay,			
ChIP - chromatin immunoprecipitation,			
FCM - flow cytometry			

Additional Comments:			
''Immunoprecipitation/Western blotting:''' Yes, very good, 17kD target			
'''Optimized conditions:''' Working dilution approx 1/500
'''Other species:''' Pretty much everything from tetrahymena to humans

[[File:1H3.jpg]] Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)