View source for Polyclonal anti-phospho-H3 - mitotic cells

Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]


'''Antibody name:''' Anti-phospho-Histone H3 (Ser10)

'''Raised in:''' rabbit

'''Subtype:''' IgG

'''Antigen:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

'''Fixatives:''' MEMFA

'''Sensitive to bleaching:''' No

'''Functions post in situ?:''' Yes

'''Immunoprecipitation/Western blotting:''' Yes, very good, 17kD target

'''Available as:''' 1 mg/ml solution. 

'''Available from:''' Upstate, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570]

'''Optimized conditions:''' Working dilution approx 1/500

'''Other species:''' Pretty much everything from tetrahymena to humans

[[File:1H3.jpg]] Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)