https://wiki.xenbase.org/xenwiki/index.php?action=history&feed=atom&title=4A6_%E2%80%93_pronephros_%28monoclonal%29 2026-11-09T21:02:16Z Revision history for this page on the wiki MediaWiki 1.44.2 https://wiki.xenbase.org/xenwiki/index.php?title=4A6_%E2%80%93_pronephros_(monoclonal)&diff=47023&oldid=prev 2011-02-04T16:45:39Z

<p>Created page with &quot;Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&amp;personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/l...&quot;</p> <p><b>New page</b></p><div>Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&amp;personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&amp;articleId=19219]<br /> <br /> <br /> &#039;&#039;&#039;Antibody name:&#039;&#039;&#039; 4A6<br /> <br /> &#039;&#039;&#039;Raised in:&#039;&#039;&#039; mouse, monoclonal<br /> <br /> &#039;&#039;&#039;Subtype:&#039;&#039;&#039; IgG<br /> <br /> &#039;&#039;&#039;Antigen:&#039;&#039;&#039; dissected pronephroi. Specific target unknown.<br /> <br /> &#039;&#039;&#039;Developmental Window:&#039;&#039;&#039; First detects target at stage 39, continues until latest stages tested.<br /> <br /> &#039;&#039;&#039;Stains:&#039;&#039;&#039; distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.<br /> <br /> &#039;&#039;&#039;Fixatives:&#039;&#039;&#039; MEMFA<br /> <br /> &#039;&#039;&#039;Sensitive to bleaching:&#039;&#039;&#039; No<br /> <br /> &#039;&#039;&#039;Functions post in situ?:&#039;&#039;&#039; No<br /> <br /> &#039;&#039;&#039;Immunoprecipitation/Western blotting:&#039;&#039;&#039; Works with both<br /> <br /> &#039;&#039;&#039;Available as:&#039;&#039;&#039; cell supernatent, 3 x<br /> <br /> &#039;&#039;&#039;Available from;&#039;&#039;&#039; Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.<br /> <br /> &#039;&#039;&#039;Optimized conditions:&#039;&#039;&#039; Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.<br /> <br /> &#039;&#039;&#039;Other species:&#039;&#039;&#039; Xenopus only<br /> <br /> References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&amp;articleId=19219]</div>

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