https://wiki.xenbase.org/xenwiki/index.php?action=history&feed=atom&title=Benzidine-peroxidase_staining_for_blood_%28Conlon_lab%29 2026-08-10T11:10:59Z Revision history for this page on the wiki MediaWiki 1.44.2 https://wiki.xenbase.org/xenwiki/index.php?title=Benzidine-peroxidase_staining_for_blood_(Conlon_lab)&diff=47319&oldid=prev 2011-11-22T14:39:36Z

<p>Created page with &quot;Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Benzid...&quot;</p> <p><b>New page</b></p><div>Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot]<br /> <br /> <br /> link to protocol document [http://www.unc.edu/~fconlon/Protocols/Benzidine%20Staining.doc]<br /> <br /> <br /> Benzidine Staining<br /> <br /> (Hemoglobin histochemical stain)<br /> <br /> 1) Make up 4X Benzidine stock<br /> <br /> :100 mg Benzidine<br /> :3.125 ml HAc (Glacial)<br /> :3.125 ml H2O<br /> :store @ 4°C in the DARK<br /> <br /> 2) Fix Embryos in 1X benzidine for 5-10 minutes<br /> :500ul Benzidine/1480ul H2O<br /> <br /> 3) Add 20ul 30% H2O2<br /> <br /> 4) Stain 2-15 minutes<br /> <br /> 5) Wash several times in 12% Glacial Acetic Acid<br /> <br /> 6) Fix 1.5 h in MEMFA<br /> <br /> 7) Change MEMFA 3X<br /> <br /> 8) Wash 3-4X EtOH<br /> <br /> 9) Store @ 4°C or 20°C in EtOH</div>

imported>Virgilio