https://wiki.xenbase.org/xenwiki/index.php?action=history&feed=atom&title=Embryo_manipulation%2Fdissection 2026-07-04T07:52:36Z Revision history for this page on the wiki MediaWiki 1.44.2 https://wiki.xenbase.org/xenwiki/index.php?title=Embryo_manipulation/dissection&diff=47385&oldid=prev 2011-12-14T15:00:49Z

<p>Created page with &quot;&#039;&#039;&#039;In this section:&#039;&#039;&#039; :Agarose Plates, 1% (for use with trops or laevis) :Dehydrating embryos :Rehydrating Embryos(for embryos stored in 100% methanol) &#039;&#039;&#039;Agarose Plates, 1% ...&quot;</p> <p><b>New page</b></p><div>&#039;&#039;&#039;In this section:&#039;&#039;&#039;<br /> :Agarose Plates, 1% (for use with trops or laevis)<br /> :Dehydrating embryos<br /> :Rehydrating Embryos(for embryos stored in 100% methanol)<br /> <br /> <br /> <br /> &#039;&#039;&#039;Agarose Plates, 1% (for use with trops or laevis)&#039;&#039;&#039;<br /> <br /> 1 g agarose powder + 100 ml 1/9 X MR without gent in 500 ml jar<br /> <br /> :make no more than 300 ml (to prevent boil over)<br /> <br /> Microwave on high 2 min/100 ml (w/ loose lid)<br /> <br /> Swirl and check for solids<br /> <br /> When completely melted, fill petri dish 1/4 full<br /> <br /> Put in 4C for 5 min, or air dry 30 min<br /> <br /> Lift out with tweezers<br /> <br /> :Can use a mold in agar before it hardens, or cut out desired shape after hardens<br /> <br /> <br /> &#039;&#039;&#039;Dehydrating embryos&#039;&#039;&#039;<br /> <br /> Embryos can be placed directly into 100% methanol in small glass vials.<br /> <br /> Embryos can be stored at -20 deg C.<br /> <br /> <br /> &#039;&#039;&#039;Rehydrating Embryos(for embryos stored in 100% methanol)&#039;&#039;&#039;<br /> <br /> Draw off methanol with vacuum<br /> <br /> Rehydrate embryos stepwise through each wash, 5 minutes each, on rotating rack:<br /> <br /> 3:1 methanol:PBS<br /> <br /> 1:1 methanol:PBS<br /> <br /> 1:3 methanol:PBS<br /> <br /> 100% PBS, repeat 2 - 3 times</div>

imported>Virgilio