imported>Xenbase at 00:22, 31 July 2021

2021-07-31T00:22:22Z

← Older revision		Revision as of 00:22, 31 July 2021
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	'''Paraformaldehyde(PFA), 20%'''		'''Paraformaldehyde (PFA), 100mls of 4%'''

	Measure 4g of Paraformaldehyde in the hood then add to 100 mls of pre-heated PBS.		Measure 4g of Paraformaldehyde in the hood then add to 100 mls of pre-heated PBS.

imported>Virgilio: Created page with "'''In this section:''' :MEMFA :Bouin’s Fixative :Dent's Fix (Methanol/DMSO) :Paraformaldehyde(PFA), 20% :PTw Containing 4% Paraformaldehyde '''MEMFA''' (MOPS/EGTA/Magnesium S..."

2011-12-12T20:42:46Z

Created page with "'''In this section:''' :MEMFA :Bouin’s Fixative :Dent's Fix (Methanol/DMSO) :Paraformaldehyde(PFA), 20% :PTw Containing 4% Paraformaldehyde '''MEMFA''' (MOPS/EGTA/Magnesium S..."

New page

'''In this section:'''
:MEMFA
:Bouin’s Fixative
:Dent's Fix (Methanol/DMSO)
:Paraformaldehyde(PFA), 20%
:PTw Containing 4% Paraformaldehyde

'''MEMFA''' (MOPS/EGTA/Magnesium Sulfate/Formaldehyde Buffer)

{|border="1" cellpadding="5" cellspacing="0" align="center"
|-
|
! scope="col" style="background:#efefef;" | 1X
! scope="col" style="background:#efefef;" | 10X
! scope="col" style="background:#efefef;" | for 1 L 10X
|-
! scope="row" style="background:#efefef;" | MOPS (pH 7.4)
|0.1 M
|1.0 M
|209.27 g
|-
! scope="row" style="background:#efefef;" | EGTA
|2 mM
|20 mM
|7.608 g
|-
! scope="row" style="background:#efefef;" | MgSO4
|1 mM
|10 mM
|2.46 g
|-
! scope="row" style="background:#efefef;" | Formaldehyde
|3.7%
|
|
|}

* Prepare a 10 X solution without the formaldehyde for storage. Add fresh formaldehyde(1/10 volume of standard 30% stock) prior to use. The solution will turn yellow with age and when autoclaved.

'''Bouin’s Fixative'''

For 50 ml:
35 ml Piric acid
12.5 ml Formaldehyde
2.5 ml glacial acetic acid
Adjust volume, store at RT.

'''Dent's Fix (Methanol/DMSO)'''

:4 volumes methanol
:1 volume DMSO

'''Paraformaldehyde(PFA), 20%'''

Measure 4g of Paraformaldehyde in the hood then add to 100 mls of pre-heated PBS.

Heat to around 62-64C.

Once dissolved aliquot 20 mls per tube and store at -20C.

'''PTw Containing 4% Paraformaldehyde'''

:1 volume of 20% paraformaldehyde
:4 volumes of PTw

Note: To made 20% paraformaldehyde in water, neutralize the cloudy solution with NaOH and then heat at 65 deg C, with shaking, until clear, Store at -20 dec C.

'''PTw'''

1X PBS(phosphate buffered saline)

0.1% Tween-20

Fixatives - Revision history

imported>Xenbase at 00:22, 31 July 2021

imported>Virgilio: Created page with "'''In this section:''' :MEMFA :Bouin’s Fixative :Dent's Fix (Methanol/DMSO) :Paraformaldehyde(PFA), 20% :PTw Containing 4% Paraformaldehyde '''MEMFA''' (MOPS/EGTA/Magnesium S..."