imported>Virgilio: Created page with "'''In this section:''' :HEPES 1 M, pH 7.4 :1M MgCl2 :10x Phosphate Buffered Saline (PBS) :20x Phosphate Buffered Saline (PBS) :PBT :Proteinase K(PK), 10 mg/ml :PTw :4M NaCl, 1 li..."

2011-12-12T20:47:59Z

Created page with "'''In this section:''' :HEPES 1 M, pH 7.4 :1M MgCl2 :10x Phosphate Buffered Saline (PBS) :20x Phosphate Buffered Saline (PBS) :PBT :Proteinase K(PK), 10 mg/ml :PTw :4M NaCl, 1 li..."

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'''In this section:'''
:HEPES 1 M, pH 7.4
:1M MgCl2
:10x Phosphate Buffered Saline (PBS)
:20x Phosphate Buffered Saline (PBS)
:PBT
:Proteinase K(PK), 10 mg/ml
:PTw
:4M NaCl, 1 liter
:SDS 10%
:TAE 50X
:1M Tris pH 9.5

'''HEPES 1 M, pH 7.4'''

For 1Liter:
:Hepes 238.31 g

Dissolve, Adjust pH and volume. Store at RT.'

'''1M MgCl2'''

For 500 ml:

Dissolve 47.606 g MgCl2 in dH2O. Adjust volume, store at RT.

'''10x Phosphate Buffered Saline (PBS)'''

10X For 4 L bottle:
:NaCl 320 g
:KCl 8 g Na2HPO4 57.6 g
:KH2PO4 9.6 g

Dissolve in dH2O. Adjust pH to 7.4, and bring to volume. Store at RT.

'''20x PBS (phosphate-buffered saline)'''

:Na2PO4 H20 - 5.12 g
"Ha2HPO4 - 23.88 g
:NaCl - 204.4 g
:distilled water - 800 ml

Adjust pH to 7.4 and volume to 1 liter.

'''PBT'''

:PBS (phosphate buffered saline)
:2 mg/ml BSA (bovine serum albumin) (Fraction 5, reagent grade)
:0.01% Triton X-100

'''Proteinase K(PK), 10 mg/ml'''

Supplied as 100 mg powder, (American Bioanalytical AB00925-000100)

Add 10 ml ddH20 for conc. of 10 mg/ml, mix until dissolved.
Divide into 150 µl aliquots, store in -20C

'''PTw'''

1X PBS(phosphate buffered saline)

0.1% Tween-20

'''4M NaCl, 1 liter'''

Dissolve 233.76 g NaCl in 750 ml dH2O. Adjust volume.

'''SDS 10%'''

For 1 L:

Dissolve 100 g SDS in 800 ml of dH2O. Adjust volume, store at RT.

'''Sodium Phosphate Buffer(1M)'''

:71 g Na2Hpo4
:4 ml H3P04
:1 Liter H20 titrate to pH 7.4

'''TAE 50X'''

For 2 L:
:Tris 484 g
:114.2 ml Glacial Acetic acid
:200 ml 0.5 M EDTA pH 8.0

Dissolve in dH2O, adjust volume. Store at RT.

'''1M Tris pH 9.5'''

For 1 L:

Dissolve 121.14 g Tris in 700 ml dH2O. Adjust pH to 9.5 with HCl Adjust volume, store at RT.

General buffers and salt solutions - Revision history

imported>Virgilio: Created page with "'''In this section:''' :HEPES 1 M, pH 7.4 :1M MgCl2 :10x Phosphate Buffered Saline (PBS) :20x Phosphate Buffered Saline (PBS) :PBT :Proteinase K(PK), 10 mg/ml :PTw :4M NaCl, 1 li..."