Monoclonal 4A6 - pronephros - Revision history

136.159.234.84 at 22:14, 14 January 2010

2010-01-14T22:14:55Z

← Older revision		Revision as of 22:14, 14 January 2010
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	'''Sensitive to bleaching:''' No		'''Sensitive to bleaching:''' No

			'''Functions post in situ?:''' No

	'''Immunoprecipitation/Western blotting:''' Works with both		'''Immunoprecipitation/Western blotting:''' Works with both

imported>Xenbase: moved Monoclonal 4A6 to Monoclonal 4A6 - pronephros

2010-01-13T00:00:12Z

moved Monoclonal 4A6 to Monoclonal 4A6 - pronephros

← Older revision	Revision as of 00:00, 13 January 2010
(No difference)

imported>Pvize at 23:42, 11 January 2010

2010-01-11T23:42:36Z

← Older revision		Revision as of 23:42, 11 January 2010
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	Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]		Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]

imported>Pvize at 23:41, 11 January 2010

2010-01-11T23:41:42Z

← Older revision		Revision as of 23:41, 11 January 2010
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	'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested.		'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested.

			'''Stains:''' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.

	'''Fixatives:''' MEMFA		'''Fixatives:''' MEMFA

imported>Pvize at 23:37, 11 January 2010

2010-01-11T23:37:49Z

← Older revision		Revision as of 23:37, 11 January 2010
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	'''Available from;''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.		'''Available from;''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.

	'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos~~. In cleared embryos both pronephroi are often in view, one in focus and one blurred, and this can make photography difficult see for example stage 32 sample above~~.		'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.

	'''Other species:''' ~~works on other anurans (Bombina), and also on axolotls when formalin plus 10% DMSO is used as the fixative, but it isn't nearly as clean as with~~ Xenopus ~~or Bombina. This is probably just a protocol problem. Reported to work on zebrafish.~~		'''Other species:''' Xenopus only

	References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]		References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]

imported>Pvize: Created page with 'Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753] '''Antibody name:''' 4A6 '''Raised in:''' mouse, mon…'

2010-01-11T23:37:00Z

Created page with 'Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753] '''Antibody name:''' 4A6 '''Raised in:''' mouse, mon…'

New page

Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]

'''Antibody name:''' 4A6

'''Raised in:''' mouse, monoclonal

'''Subtype:''' IgG

'''Antigen:''' dissected pronephroi. Specific target unknown.

'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested.

'''Fixatives:''' MEMFA

'''Sensitive to bleaching:''' No

'''Immunoprecipitation/Western blotting:''' Works with both

'''Available as:''' cell supernatent, 3 x

'''Available from;''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.

'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. In cleared embryos both pronephroi are often in view, one in focus and one blurred, and this can make photography difficult see for example stage 32 sample above.

'''Other species:''' works on other anurans (Bombina), and also on axolotls when formalin plus 10% DMSO is used as the fixative, but it isn't nearly as clean as with Xenopus or Bombina. This is probably just a protocol problem. Reported to work on zebrafish.

References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]