Protocols - Revision history

Xenbase: /* Gene Editing */

2026-08-17T19:07:34Z

Gene Editing

← Older revision		Revision as of 19:07, 17 August 2026
Line 139:		Line 139:

	=== '''''Gene Editing''''' ===		=== '''''Gene Editing''''' ===
	**'''Protocol for blastomere injection to generate bilateral hemimosaic Xenopus tadpoles.'''-Li et al 2026. STAR Protocols [https://www.sciencedirect.com/science/article/pii/S2666166726000407?via%3Dihub]		**'''Protocol for blastomere injection to generate bilateral hemimosaic Xenopus tadpoles.'''Li et al 2026 (Ed Ruthazer lab). STAR Protocols [https://www.sciencedirect.com/science/article/pii/S2666166726000407?via%3Dihub]
	**'''Generation and Analysis of ''Xenopus laevis'' Models of Retinal Degeneration Using CRISPR/Cas9''' Feehan ''et al'' 2019. (Moritz lab, University of British Columbia). MiMB, vol 1834. [https://doi.org/10.1007/978-1-4939-8669-9_14]		**'''Generation and Analysis of ''Xenopus laevis'' Models of Retinal Degeneration Using CRISPR/Cas9''' Feehan ''et al'' 2019. (Moritz lab, University of British Columbia). MiMB, vol 1834. [https://doi.org/10.1007/978-1-4939-8669-9_14]
	**'''Step-by-Step Protocol for Making a Knock-In Xenopus laevis to Visualize Endogenous Gene Expression.''' Norie Kagawa, Yoshihiko Umesono, Ken-ichi T. Suzuki, Makoto Mochii. 2025. Dev Growth & Diff. [ https://doi.org/10.1111/dgd.70011]		**'''Step-by-Step Protocol for Making a Knock-In Xenopus laevis to Visualize Endogenous Gene Expression.''' Norie Kagawa, Yoshihiko Umesono, Ken-ichi T. Suzuki, Makoto Mochii. 2025. Dev Growth & Diff. [ https://doi.org/10.1111/dgd.70011]

Xenbase: /* Gene Editing */

2026-08-17T19:05:35Z

Gene Editing

← Older revision		Revision as of 19:05, 17 August 2026
Line 139:		Line 139:

	=== '''''Gene Editing''''' ===		=== '''''Gene Editing''''' ===
			**'''Protocol for blastomere injection to generate bilateral hemimosaic Xenopus tadpoles.'''-Li et al 2026. STAR Protocols [https://www.sciencedirect.com/science/article/pii/S2666166726000407?via%3Dihub]
	**'''Generation and Analysis of ''Xenopus laevis'' Models of Retinal Degeneration Using CRISPR/Cas9''' Feehan ''et al'' 2019. (Moritz lab, University of British Columbia). MiMB, vol 1834. [https://doi.org/10.1007/978-1-4939-8669-9_14]		**'''Generation and Analysis of ''Xenopus laevis'' Models of Retinal Degeneration Using CRISPR/Cas9''' Feehan ''et al'' 2019. (Moritz lab, University of British Columbia). MiMB, vol 1834. [https://doi.org/10.1007/978-1-4939-8669-9_14]
	**'''Step-by-Step Protocol for Making a Knock-In Xenopus laevis to Visualize Endogenous Gene Expression.''' Norie Kagawa, Yoshihiko Umesono, Ken-ichi T. Suzuki, Makoto Mochii. 2025. Dev Growth & Diff. [ https://doi.org/10.1111/dgd.70011]		**'''Step-by-Step Protocol for Making a Knock-In Xenopus laevis to Visualize Endogenous Gene Expression.''' Norie Kagawa, Yoshihiko Umesono, Ken-ichi T. Suzuki, Makoto Mochii. 2025. Dev Growth & Diff. [ https://doi.org/10.1111/dgd.70011]

Xenbase: /* Transgenics */

2026-08-17T19:04:55Z

Transgenics

← Older revision		Revision as of 19:04, 17 August 2026
Line 64:		Line 64:

	==='''''Transgenics'''''===		==='''''Transgenics'''''===
	**'''Protocol for blastomere injection to generate bilateral hemimosaic Xenopus tadpoles.'''-Li et al 2026. STAR Protocols [https://www.sciencedirect.com/science/article/pii/S2666166726000407?via%3Dihub]
	**'''Transgenic ''Xenopus laevis'' by Restriction Enzyme Mediated Integration and Nuclear Transplantation''' - Amaya & Kroll [http://www.jove.com/video/2010/production-transgenic-xenopus-laevis-restriction-enzyme-mediated]		**'''Transgenic ''Xenopus laevis'' by Restriction Enzyme Mediated Integration and Nuclear Transplantation''' - Amaya & Kroll [http://www.jove.com/video/2010/production-transgenic-xenopus-laevis-restriction-enzyme-mediated]

Xenbase: /* Transgenics */

2026-08-17T19:01:57Z

Transgenics

← Older revision		Revision as of 19:01, 17 August 2026
Line 64:		Line 64:

	==='''''Transgenics'''''===		==='''''Transgenics'''''===
			**'''Protocol for blastomere injection to generate bilateral hemimosaic Xenopus tadpoles.'''-Li et al 2026. STAR Protocols [https://www.sciencedirect.com/science/article/pii/S2666166726000407?via%3Dihub]
	**'''Transgenic ''Xenopus laevis'' by Restriction Enzyme Mediated Integration and Nuclear Transplantation''' - Amaya & Kroll [http://www.jove.com/video/2010/production-transgenic-xenopus-laevis-restriction-enzyme-mediated]		**'''Transgenic ''Xenopus laevis'' by Restriction Enzyme Mediated Integration and Nuclear Transplantation''' - Amaya & Kroll [http://www.jove.com/video/2010/production-transgenic-xenopus-laevis-restriction-enzyme-mediated]

Xenbase: /* MassSpec & Proteomics */

2026-07-29T19:58:50Z

MassSpec & Proteomics

← Older revision		Revision as of 19:58, 29 July 2026
Line 152:		Line 152:

	=== '''''MassSpec & Proteomics''''' ===		=== '''''MassSpec & Proteomics''''' ===
	** '''Quantitative Proteomics of ''Xenopus'' Embryos I, Sample Preparation'''. Meera Gupta, Matthew Sonnett, Lillia Ryazanova, Marc Presler, Martin Wühr . 2018. Methods Mol Biol 2018;1865:175-194. [[doi: 10.1007/978-1-4939-8784-9_13]]		**'''Cleland immunoblotting quantifies cysteine proteoform ensembles.'''. James Cobly, Anna Noble & Matt Guille (EXRC). 2026. Free Radic Biol Med. [https://doi.org/10.1016/j.freeradbiomed.2026.04.026]
			** '''Quantitative Proteomics of ''Xenopus'' Embryos I, Sample Preparation'''. Meera Gupta, Matthew Sonnett, Lillia Ryazanova, Marc Presler, Martin Wühr . 2018. Methods Mol Biol 2018;1865:175-194.[https://doi.org/10.1007/978-1-4939-8784-9_13]
	**'''High-throughput transporter identification for small molecules in cell factories with validation using ''Xenopus'' expression assays.''' See ''Transportome-wide engineering of Saccharomyces cerevisiae,''. 2021. Wang et al. Metabolic Engineering.[https://www.sciencedirect.com/science/article/pii/S109671762100015X?via%3Dihub]		**'''High-throughput transporter identification for small molecules in cell factories with validation using ''Xenopus'' expression assays.''' See ''Transportome-wide engineering of Saccharomyces cerevisiae,''. 2021. Wang et al. Metabolic Engineering.[https://www.sciencedirect.com/science/article/pii/S109671762100015X?via%3Dihub]
	**'''Uptake Assays in Xenopus laevis Oocytes Using Liquid Chromatography-mass Spectrometry to Detect Transport Activity''' Jorgenson et al 2021. bio-protocol. [https://bio-protocol.org/e2581]		**'''Uptake Assays in Xenopus laevis Oocytes Using Liquid Chromatography-mass Spectrometry to Detect Transport Activity''' Jorgenson et al 2021. bio-protocol. [https://bio-protocol.org/e2581]

Xenbase: /* Animal Handling & Oocyte Harvesting */

2026-07-23T11:35:59Z

Animal Handling & Oocyte Harvesting

← Older revision		Revision as of 11:35, 23 July 2026
Line 58:		Line 58:

	==='''''Animal Handling & Oocyte Harvesting'''''===		==='''''Animal Handling & Oocyte Harvesting'''''===
			**'''Unilateral Gonadectomy in Adult Male ''Xenopus laevis''.''' -Adjartey, Ryerson, Jones & Merzdorf.2026. [https://www.jove.com/t/71258/unilateral-gonadectomy-in-adult-male-xenopus-laevis]
	**'''Obtaining Eggs from ''Xenopus laevis'' Females''' - Cross & Powers [http://www.jove.com/video/890/obtaining-eggs-from-xenopus-laevis-females]		**'''Obtaining Eggs from ''Xenopus laevis'' Females''' - Cross & Powers [http://www.jove.com/video/890/obtaining-eggs-from-xenopus-laevis-females]
	**'''Fertilization of ''Xenopus'' oocytes, Host Transfer Method''' - Schneider et al. [http://www.jove.com/video/1864/fertilization-of-xenopus-oocytes-using-the-host-transfer-method]		**'''Fertilization of ''Xenopus'' oocytes, Host Transfer Method''' - Schneider et al. [http://www.jove.com/video/1864/fertilization-of-xenopus-oocytes-using-the-host-transfer-method]

Xenbase: /* Proteomics & Transcriptomics */

2026-04-16T20:42:37Z

Proteomics & Transcriptomics

← Older revision		Revision as of 20:42, 16 April 2026
Line 41:		Line 41:

	==='''''Proteomics & Transcriptomics'''''===		==='''''Proteomics & Transcriptomics'''''===
	**'''Metabolic Labeling of the Nascent Transcriptome in Xenopus Early Embryogenesis.''' Chuan Qin & Hui Chen, University of South Carolina (2026) [DOI:10.3791/70591]		**'''Metabolic Labeling of the Nascent Transcriptome in Xenopus Early Embryogenesis.''' Chuan Qin & Hui Chen, University of South Carolina (2026) [DOI:10.3791/70591] [https://app.jove.com/t/70591/metabolic-labeling-nascent-transcriptome-xenopus-early]
	**'''Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting.''' Kanzler & Sheets (2025) [DOI: 10.3791/69139]		**'''Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting.''' Kanzler & Sheets (2025) [DOI: 10.3791/69139]

Xenbase: /* Proteomics */

2026-04-16T20:41:18Z

Proteomics

← Older revision		Revision as of 20:41, 16 April 2026
Line 40:		Line 40:
	**'''Isolating Single Cells from ''Xenopus'' Early Embryos and Sorting Them by Size'''. Chuan Qin and Hui Chen (2026) University of South Carolina. [DOI: 10.3791/70630]		**'''Isolating Single Cells from ''Xenopus'' Early Embryos and Sorting Them by Size'''. Chuan Qin and Hui Chen (2026) University of South Carolina. [DOI: 10.3791/70630]

	==='''''Proteomics'''''===		==='''''Proteomics & Transcriptomics'''''===
			**'''Metabolic Labeling of the Nascent Transcriptome in Xenopus Early Embryogenesis.''' Chuan Qin & Hui Chen, University of South Carolina (2026) [DOI:10.3791/70591]
	**'''Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting.''' Kanzler & Sheets (2025) [DOI: 10.3791/69139]		**'''Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting.''' Kanzler & Sheets (2025) [DOI: 10.3791/69139]

Xenbase: /* Proteomics */

2026-04-13T18:48:52Z

Proteomics

← Older revision		Revision as of 18:48, 13 April 2026
Line 40:		Line 40:
	**'''Isolating Single Cells from ''Xenopus'' Early Embryos and Sorting Them by Size'''. Chuan Qin and Hui Chen (2026) University of South Carolina. [DOI: 10.3791/70630]		**'''Isolating Single Cells from ''Xenopus'' Early Embryos and Sorting Them by Size'''. Chuan Qin and Hui Chen (2026) University of South Carolina. [DOI: 10.3791/70630]

	==='''Proteomics'''===		==='''''Proteomics'''''===
	**'''Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting.''' Kanzler & Sheets (2025) [DOI: 10.3791/69139]		**'''Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting.''' Kanzler & Sheets (2025) [DOI: 10.3791/69139]

Xenbase: /* Single Cell */

2026-04-13T18:48:38Z

Single Cell

← Older revision		Revision as of 18:48, 13 April 2026
Line 37:		Line 37:
	'''Journal of Visualized Experiments (JOVE) provides excellent video demonstrations and detailed protocols for a wide variety of lab protocols using ''Xenopus''. [http://www.jove.com/keyword/xenopus+embryo]		'''Journal of Visualized Experiments (JOVE) provides excellent video demonstrations and detailed protocols for a wide variety of lab protocols using ''Xenopus''. [http://www.jove.com/keyword/xenopus+embryo]

	==='''''Single ~~CelL~~'''''===		==='''''Single Cell'''''===
	**'''Isolating Single Cells from ''Xenopus'' Early Embryos and Sorting Them by Size'''. Chuan Qin and Hui Chen (2026) University of South Carolina. [DOI: 10.3791/70630]		**'''Isolating Single Cells from ''Xenopus'' Early Embryos and Sorting Them by Size'''. Chuan Qin and Hui Chen (2026) University of South Carolina. [DOI: 10.3791/70630]