C-cadherin - cdh3: Difference between revisions
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'''Antigen:''' C-cadherin | '''Antigen:''' C-cadherin | ||
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=482276 cdh3] | |||
'''Epitope:''' C-cadherin extracellular domain fused to GST | '''Epitope:''' C-cadherin extracellular domain fused to GST | ||
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'''Applications:''' IF | '''Applications:''' IF | ||
'''Antibody Name:''' | '''Antibody Name/Catalog Number:''' [http://dshb.biology.uiowa.edu/cadherin-C- 6B6] | ||
'''Supplier:''' Developmental Studies Hybridoma Bank | '''Supplier:''' Developmental Studies Hybridoma Bank | ||
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'''Comments:''' IF: directly fix in Dent's 2 hrs @ RT then to 100% methanol use at 1:300 | '''Comments:''' IF: directly fix in Dent's 2 hrs @ RT then to 100% methanol use at 1:300 | ||
'''Contributor:''' Zorn Lab and V | '''Contributor:''' Zorn Lab and V Ponferrada | ||
'''Additional Information:''' MEMFA, 10% TCA, and 4% PFA in 1xPBS fixatives also work | '''Additional Information:''' MEMFA, 10% TCA, and 4% PFA in 1xPBS fixatives also work | ||
'''Reference(s):''' | '''Reference(s):''' | ||
Latest revision as of 14:32, 8 March 2011
Antigen: C-cadherin
Xenbase Gene Page: cdh3
Epitope: C-cadherin extracellular domain fused to GST
Host Species/Isotype: mouse monoclonal
Antigen Species: xenopus
Applications: IF
Antibody Name/Catalog Number: 6B6
Supplier: Developmental Studies Hybridoma Bank
Comments: IF: directly fix in Dent's 2 hrs @ RT then to 100% methanol use at 1:300
Contributor: Zorn Lab and V Ponferrada
Additional Information: MEMFA, 10% TCA, and 4% PFA in 1xPBS fixatives also work
Reference(s):
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry