Antibody template: Difference between revisions

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Alpha-tubulin antibody for labeling microtubules
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'''Antibody name:''' DM1A
'''Antigen:'''


'''Raised in:''' Mouse
'''Epitope:'''


'''Subtype:''' IgG
'''Host Species/Isotype:'''


'''Antigen:''' Microtubules from chick embryo brain
'''Antigen Species:'''


'''Fixatives:''' Works well in MEMFA or Dent's
'''Applications:'''


'''Sensitive to bleaching:''' Yes
'''Antibody Name:'''


'''Functions post in situ?:''' Yes
'''Supplier:'''


'''Immunoprecipitation/Western blotting:'''  
'''Comments:'''


'''Available as:''' Ascites
'''Contributor:'''


'''Available from:''' Sigma (cat#T9026)


'''Optimized conditions:''' We dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room tempp for four hours.
applications abbreviations:
 
WB - Western blotting,
'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry

Latest revision as of 12:03, 4 February 2011

To use this template, click the "edit" tab, and copy the fields below into the new page you wish to create.


Antigen:

Epitope:

Host Species/Isotype:

Antigen Species:

Applications:

Antibody Name:

Supplier:

Comments:

Contributor:


applications abbreviations: WB - Western blotting, IP - immunoprecipitation, IF - immunofluorescence, IHC - immunohistochemistry, EMSA - electromobility shift assay, ChIP - chromatin immunoprecipitation, FCM - flow cytometry

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