Monoclonal 4A6 - pronephros: Difference between revisions
imported>Pvize Created page with 'Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753] '''Antibody name:''' 4A6 '''Raised in:''' mouse, mon…' |
No edit summary |
||
| (4 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753] | Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219] | ||
| Line 11: | Line 11: | ||
'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested. | '''Developmental Window:''' First detects target at stage 39, continues until latest stages tested. | ||
'''Stains:''' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias. | |||
'''Fixatives:''' MEMFA | '''Fixatives:''' MEMFA | ||
'''Sensitive to bleaching:''' No | '''Sensitive to bleaching:''' No | ||
'''Functions post in situ?:''' No | |||
'''Immunoprecipitation/Western blotting:''' Works with both | '''Immunoprecipitation/Western blotting:''' Works with both | ||
| Line 22: | Line 26: | ||
'''Available from;''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs. | '''Available from;''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs. | ||
'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos | '''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. | ||
'''Other species:''' | '''Other species:''' Xenopus only | ||
References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219] | References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219] | ||
Latest revision as of 15:14, 14 January 2010
Generated and characterized by Elizabeth Jones [1]. References: Dev Bio 171:531- [2]
Antibody name: 4A6
Raised in: mouse, monoclonal
Subtype: IgG
Antigen: dissected pronephroi. Specific target unknown.
Developmental Window: First detects target at stage 39, continues until latest stages tested.
Stains: distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.
Fixatives: MEMFA
Sensitive to bleaching: No
Functions post in situ?: No
Immunoprecipitation/Western blotting: Works with both
Available as: cell supernatent, 3 x
Available from; Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.
Optimized conditions: Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.
Other species: Xenopus only
References: Dev Bio 171:531- [3]