12/101 – somites (monoclonal): Difference between revisions
Created page with "Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article...." |
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Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article.do?method=display&articleId=40901] | '''Antigen:''' homogenized tissue | ||
'''Epitope:''' | |||
'''Host Species/Isotype:''' mouse monoclonal IgG | |||
'''Antigen Species:''' newt | |||
'''Applications:''' IF, WB | |||
'''Antibody Name:''' 12/101 | |||
'''Supplier:''' Developmental Studies Hybridoma Bank | |||
'''Comments:''' WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit) | |||
'''Contributor:''' Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article.do?method=display&articleId=40901] | |||
applications abbreviations: | |||
WB - Western blotting, | |||
IP - immunoprecipitation, | |||
IF - immunofluorescence, | |||
IHC - immunohistochemistry, | |||
EMSA - electromobility shift assay, | |||
ChIP - chromatin immunoprecipitation, | |||
FCM - flow cytometry | |||
[[File:12101.jpg]] | |||
Immunofluorescence using 12/101 | |||
[[File:12101.jpg]] | |||
Immunofluorescence using 12/101 | |||
[[File:12101+ATPase.jpg]] | [[File:12101+ATPase.jpg]] | ||
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green. | Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green. | ||
Latest revision as of 12:14, 4 February 2011
Antigen: homogenized tissue
Epitope:
Host Species/Isotype: mouse monoclonal IgG
Antigen Species: newt
Applications: IF, WB
Antibody Name: 12/101
Supplier: Developmental Studies Hybridoma Bank
Comments: WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)
Contributor: Generated and characterized by Kintner [1] and Brockes (1985) [2]
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry
Immunofluorescence using 12/101
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.