Anti-FLAG M2 monoclonal antibody: Difference between revisions
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Created page with "'''Antigen:''' FLAG epitope '''Epitope:''' aa DYKDDDDK '''Host Species/Isotype:''' mouse monoclonal '''Antigen Species:''' synthetic peptide '''Applications:''' ..." |
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'''Epitope:''' aa DYKDDDDK | '''Epitope:''' aa DYKDDDDK | ||
'''Host Species/Isotype:''' mouse monoclonal | '''Host Species/Isotype:''' mouse monoclonal, IgG1 | ||
'''Antigen Species:''' synthetic peptide | '''Antigen Species:''' synthetic peptide | ||
Revision as of 14:34, 8 March 2011
Antigen: FLAG epitope
Epitope: aa DYKDDDDK
Host Species/Isotype: mouse monoclonal, IgG1
Antigen Species: synthetic peptide
Applications: IP, IF, WB
Antibody Name: F3165 [1]
Supplier: Sigma
Comments: IP: use 2 ug, IF: use at 1:1000, WB use at 1:5000 to 1:10,000
Contributor: Zorn Lab and V Ponferrada
Additional Information: IF: MEMFA, 10% TCA fixatives work
Reference(s):
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry