Anti-FLAG M2 monoclonal antibody: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
'''Antigen:''' FLAG epitope | '''Antigen:''' FLAG epitope | ||
'''Xenbase Gene Page:''' | |||
'''Epitope:''' aa DYKDDDDK | '''Epitope:''' aa DYKDDDDK | ||
'''Host Species/Isotype:''' mouse monoclonal | '''Host Species/Isotype:''' mouse monoclonal | ||
'''Antigen Species:''' synthetic peptide | '''Antigen Species:''' synthetic peptide | ||
| Line 9: | Line 11: | ||
'''Applications:''' IP, IF, WB | '''Applications:''' IP, IF, WB | ||
'''Antibody Name:''' | '''Antibody Name/Catalog Number:''' [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=F3165|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC F3165] | ||
'''Supplier:''' Sigma | '''Supplier:''' Sigma | ||
| Line 20: | Line 22: | ||
'''Reference(s):''' | '''Reference(s):''' | ||
Latest revision as of 14:52, 8 March 2011
Antigen: FLAG epitope
Xenbase Gene Page:
Epitope: aa DYKDDDDK
Host Species/Isotype: mouse monoclonal
Antigen Species: synthetic peptide
Applications: IP, IF, WB
Antibody Name/Catalog Number: F3165
Supplier: Sigma
Comments: IP: use 2 ug, IF: use at 1:1000, WB use at 1:5000 to 1:10,000
Contributor: Zorn Lab and V Ponferrada
Additional Information: IF: MEMFA, 10% TCA fixatives work
Reference(s):
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry