Protocols: Difference between revisions
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*'''''Transgenics''''' | *'''''Transgenics''''' | ||
*[[transgenesis in Xenopus laevis - adapted from Amaya et al 2001 (Conlon lab)]] | **[[transgenesis in Xenopus laevis - adapted from Amaya et al 2001 (Conlon lab)]] | ||
| Line 54: | Line 54: | ||
**[[benzidine-peroxidase staining for blood (Thomsen lab)]] | **[[benzidine-peroxidase staining for blood (Thomsen lab)]] | ||
**[[apoptosis via TUNEL (Veenstra lab)]] | **[[apoptosis via TUNEL (Veenstra lab)]] | ||
**[[TUNEL staining of whole Xenopus embryos - Harland protocol (Conlon lab)]] | |||
| Line 63: | Line 61: | ||
**[[silver staining protein gels (Stukenberg lab)]] | **[[silver staining protein gels (Stukenberg lab)]] | ||
**[[immunofluorescence with biotinylated antibodies (Stukenberg lab)]] | **[[immunofluorescence with biotinylated antibodies (Stukenberg lab)]] | ||
**Purifying Polyclonal Antibodies using Affinity Columns/Chromatography | |||
***[[Making an Affinity Column]] | |||
***[[Running an Affinity Column]] | |||
Revision as of 08:46, 22 November 2011
- Books for Xenopus Research and Protocols
- Normal Table of Xenopus Laevis (Daudin), Nieuwkoop and Faber (Third edition), 1994 [1]
- a systematic and chronological description of Xenopus laevis development
- Early Development of Xenopus laevis: A Laboratory Manual, Sive, Grainger, and Harland, 2000 [2]
- comprehensive collection of protocols for the study of early development in Xenopus embryos
- Xenopus Protocols: Cell Biology and Signal Transduction, Liu (First edition), 2006 [3]
- step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents
- Xenopus laevis: Practical Uses in Cell and Molecular Biology, Volume 36, Kay and Peng, 1991 [4]
- detailed description of a wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology
- Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology, Seidman and Soreq, 1997 [5]
- referenced guide to the use of microinjected embryos studying the role and regulation of nervous system proteins during development
- Atlas of Xenopus Development, Bernardini, Prati, Bonetti, and Scari, 1999 [6]
- scanning, transmission, and light microscopy images of Xenopus embryonic development
- The early development of Xenopus laevis. An atlas of the histology, Hausen and Riebesell, 1991 [7]
- detailed histological sections of Xenopus embryonic development
- Color Atlas of Xenopus laevis Histology, Wiechmann and Wirsig-Wiechmann, 2003 [8]
- central source on the microscopic anatomy of cells, tissues, and major organs of Xenopus laevis
- Normal Table of Xenopus Laevis (Daudin), Nieuwkoop and Faber (Third edition), 1994 [1]
- Animal Husbandry/Animal Protocols
- In Situ Hybridization
- Immunohisochemistry
- Other Embryo Staining Protocols
- Other Immuno Protocols
- immunoprecipitation (Stukenberg lab)
- silver staining protein gels (Stukenberg lab)
- immunofluorescence with biotinylated antibodies (Stukenberg lab)
- Purifying Polyclonal Antibodies using Affinity Columns/Chromatography
- Xenopus Oocyte and Egg Extracts
- crude interphase egg extract (Shechter lab)
- isolation of chromatin from egg extract and histone recovery (Shechter lab)
- preparation of cycling egg extract (Shechter lab)
- cycling extracts, mike's protocol (Stukenberg lab)
- cycling extracts, sara's protocol (Stukenberg lab)
- CSF extract, mike's protocol (Stukenberg lab)
- CSF extract, sara's protocol (Stukenberg lab)
- assaying H1 kinase in extracts (Stukenberg lab)