Lab Solutions and Reagents: Difference between revisions
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**dNTPs 10 mM | **dNTPs 10 mM | ||
**2.5% Ficoll | **2.5% Ficoll | ||
**2.5% Ficoll in 1/3X MR (for use with X. laevis embryos) | |||
**3% Ficoll in 1/9XMR (for use with X.tropicalis embryos) | **3% Ficoll in 1/9XMR (for use with X.tropicalis embryos) | ||
**gentamicin stock, Sigma (1000X, 100 mg/ml) | **gentamicin stock, Sigma (1000X, 100 mg/ml) | ||
| Line 22: | Line 23: | ||
**modified Ringers | **modified Ringers | ||
**4M NaCl, 1 liter | **4M NaCl, 1 liter | ||
**phosphate buffered saline(PBS), 10X | **paraformaldehyde(PFA), 20% | ||
**phosphate buffered saline (PBS), 10X | |||
**proteinase K(PK), 10 mg/ml | **proteinase K(PK), 10 mg/ml | ||
**PTw | **PTw | ||
| Line 31: | Line 33: | ||
**SSC, 20X | **SSC, 20X | ||
**TAE 50X | **TAE 50X | ||
**1M Tris pH 9.5 | |||
*[[benzocaine stock, 100x (Khokha lab)]] | *[[benzocaine stock, 100x (Khokha lab)]] | ||
*[[high-salt solution for egg laying (Zorn lab/Ron Laskey)]] | *[[high-salt solution for egg laying (Zorn lab/Ron Laskey)]] | ||
Revision as of 12:22, 6 December 2011
- recipes for miscellaneous salts, solutions, etc. (Khokha lab)
- agarose plates 1% - for use with laevis or tropicalis
- amphibian Ringers, 5 gal (19 L)
- bleaching solution (Sive et al. 2000, Mayor et al. 1995)
- Bouin’s fixative
- cysteine, 2.0%
- dehydrating embryos
- dNTPs 10 mM
- 2.5% Ficoll
- 2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)
- 3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)
- gentamicin stock, Sigma (1000X, 100 mg/ml)
- HEPES 1 M, pH 7.4
- hybridization buffer
- levamisol 1 M
- MAB, 10X
- 1M MgCl2
- MEMFA* (Sive et al. 2000)
- 100X MR salts
- 1/3X Modified Ringers
- 1/9X MR + 1% Gentamicin
- 1/20X MR
- modified Ringers
- 4M NaCl, 1 liter
- paraformaldehyde(PFA), 20%
- phosphate buffered saline (PBS), 10X
- proteinase K(PK), 10 mg/ml
- PTw
- rehydrating embryos (for embryos stored in 100% methanol)
- RNase A (Sigma R 5000)
- RNase T1 (Sigma R8251)
- SDS 10%
- SSC, 20X
- TAE 50X
- 1M Tris pH 9.5