Lab Solutions and Reagents

Please click each heading to see solution/reagent recipes

• culture media

Modified Barth's Saline (MBS)

Anitbiotics

Gentamycin

Oxytetracycline

Penicillin

Streptomycin Sulfate

High-salt MBS

Amphibian Ringers

Modified Frog Ringers (MR)

Marc's Modified Ringer's (MMR)

Normal Amphibian Medium (NAM)

Holtfreter's Solution

• immunohistochemistry
• in situ hybridization
• fixatives
• DNA/RNA preparation
• embryo manipulation/dissection
• microinjection
• general buffers and salt solutions

• benzocaine stock, 100x (Khokha lab)

• high-salt solution for egg laying (Zorn lab/Ron Laskey)

• recipes for miscellaneous salts, solutions, etc. (Khokha lab)
  • agarose plates 1% - for use with laevis or tropicalis
  • Alkaline Phosphatase (AP) Buffer, with Levamisol and Tween
  • amphibian Ringers, 5 gal (19 L)
  • bleaching solution (Sive et al. 2000, Mayor et al. 1995)
  • 4% BMB blocking reagent in MAB
  • Bouin’s fixative
  • cysteine, 2.0%
  • dehydrating embryos
  • Denhardt’s solution, 100X
  • dNTPs 10 mM
  • 2.5% Ficoll
  • 2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)
  • 3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)
  • gentamicin stock, Sigma (1000X, 100 mg/ml)
  • HEPES 1 M, pH 7.4
  • hybridization buffer
  • levamisol 1 M
  • MAB, 10X
  • 1M MgCl2
  • MEMFA* (Sive et al. 2000)
  • 100X MR salts
  • 1/3X Modified Ringers
  • 1/9X MR + 1% Gentamicin
  • 1/20X MR
  • modified Ringers
  • paraformaldehyde(PFA), 20%
  • phosphate buffered saline (PBS), 10X
  • proteinase K(PK), 10 mg/ml
  • PTw
  • rehydrating embryos (for embryos stored in 100% methanol)
  • RNase A (Sigma R 5000)
  • RNase T1 (Sigma R8251)
  • 4M NaCl, 1 liter
  • SDS 10%
  • Sodium Phosphate Buffer(1M)
  • SSC, 20X
  • TAE 50X
  • Triethanolamine 0.1 M (pH7.0-8.0)
  • 1M Tris pH 9.5