Antibody template: Difference between revisions

Revision as of 10:07, 16 June 2010

Alpha-tubulin antibody for labeling microtubules

Antibody name: DM1A

Raised in: Mouse

Subtype: IgG

Antigen: Microtubules from chick embryo brain

Fixatives: Works well in MEMFA or Dent's

Sensitive to bleaching: Yes

Functions post in situ?: Yes

Immunoprecipitation/Western blotting:

Available as: Ascites

Available from: Sigma (cat#T9026)

Optimized conditions: We dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room tempp for four hours.

Other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse

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-Generated and characterized by XXX [http://www.xenbase.org:80/community/person.do?method=display&personId=XXX]
+Alpha-tubulin antibody for labeling microtubules
-'''Antibody name:''' XXX
+'''Antibody name:''' DM1A
-'''Raised in:''' XXX
+'''Raised in:''' Mouse
-'''Subtype:''' IgX
+'''Subtype:''' IgG
-'''Antigen:''' XXX
+'''Antigen:''' Microtubules from chick embryo brain
-'''Fixatives:'''
+'''Fixatives:''' Works well in MEMFA or Dent's
-'''Sensitive to bleaching:''' Yes/No
+'''Sensitive to bleaching:''' Yes
-'''Functions post in situ?:''' Yes/No
+'''Functions post in situ?:''' Yes
 '''Immunoprecipitation/Western blotting:'''
-'''Available as:''' cell supernatant, 3 x
+'''Available as:''' Ascites
-'''Available from:'''
+'''Available from:''' Sigma (cat#T9026)
-'''Optimized conditions:'''
+'''Optimized conditions:''' We dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room tempp for four hours.
-'''Other species:'''
+'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse