LEL - all pronephric epithelia- also faint lateral line: Difference between revisions
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Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples. | Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples. | ||
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[[File:FLEL.jpg]] (Image from P. Vize [http://www.xenbase.org/community/person.do?method=display&personId=702&tabId=0])]] | |||
[[Image:LEL_4.jpg|thumb|alt=ALT TEXT|Stage 38/39 Xenopus laevis embryos stained with fluorescein conjugated LEL (Image from P. Vize [http://www.xenbase.org/community/person.do?method=display&personId=702&tabId=0])]] | [[Image:LEL_4.jpg|thumb|alt=ALT TEXT|Stage 38/39 Xenopus laevis embryos stained with fluorescein conjugated LEL (Image from P. Vize [http://www.xenbase.org/community/person.do?method=display&personId=702&tabId=0])]] |
Revision as of 16:33, 14 January 2010
Lycopersicon Esculentum (Tomato) Lectin (LEL)
Both Fluorescein (FL-1171 ) and Texas Red (TL-1176) conjugates are available from Vector Laboratories [2]
Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples.
(Image from P. Vize [3])]]