4A6 – pronephros
Antigen: dissected pronephroi, target unknown
Xenbase Gene Page:
Epitope: mouse monoclonal IgG
Host Species/Isotype:
Antigen Species:
Applications: IF, IP, WB
Antibody Name/Catalog Number: 4A6
Supplier: Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.
Comments: not sentitive to bleaching, does not function post in situ
Contributor: Generated and characterized by Elizabeth Jones [1]. References: Dev Bio 171:531- [2]
Additional Information:
Developmental Window: First detects target at stage 39, continues until latest stages tested.
Stains:' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.
Fixatives: MEMFA
Available as:' cell supernatant, 3 x
Optimized conditions: Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.
Other species: Xenopus only
Reference(s): Dev Bio 171:531- [3]
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry