Lab Solutions and Reagents
most information adapted from:
Early Development of Xenopus Laevis: A Laboratory Manual.[1]
2000; (First ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
Sive, Hazel (Author), Grainger, Robert M (Author), and Harland, Richard M (Author).
Additional methodology adapted from Khokha lab protocols [2]
click each bulleted list heading to view solution/reagent recipes
- Modified Barth's Saline (MBS)
- Anitbiotics
- Gentamycin
- Oxytetracycline
- Penicillin
- Streptomycin Sulfate
- High-salt MBS
- Amphibian Ringers
- Modified Frog Ringers (MR)
- Marc's Modified Ringer's (MMR)
- Normal Amphibian Medium (NAM)
- Holtfreter's Solution
- MEMFA
- Dent's Fix (Methanol/DMSO)
- Bleaching solution (Mayor et al. 1995)
- Goat Serum (GS)
- PBT
- DAB
- H2O2
- Secondary Antibodies Coupled to Horseradish Peroxidase
- Murray's Clearing Medium
- Nucleotide Stocks
- 5x SP6 Buffer
- MEMFA
- PTw
- Triethanolamine 0.1 M
- PTw Containing 4% Paraformaldehyde
- Hybridization Buffer
- SSC, 20X
- Denhardt’s solution, 100X
- RNase A
- RNase T1
- 4% BMB Blocking Reagent in MAB
- Alkaline Phosphatase Buffer
- Levamisol 1 M
- NBT/BCIP
- PVA Alkaline Phosphatase Buffer
- Bouin’s Fixative
- Bleaching solution (Mayor et al. 1995)
- MEMFA
- Bouin’s Fixative
- Dent's Fix (Methanol/DMSO)
- Paraformaldehyde(PFA), 20%
- PTw Containing 4% Paraformaldehyde
- 10x mRNA Transcription Buffer
- 2x mRNA Nucleotide Triphosphate Mix
- TE
- Homoginization Buffer
- Reticulocyte Standard Buffer (RSB)
- Nuclei Freezing Buffer
- Agarose Plates, 1% (for use with trops or laevis)
- Dehydrating embryos
- Rehydrating Embryos(for embryos stored in 100% methanol)
- Ficoll, 2.5%
- 2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)
- 3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)
- HEPES 1 M, pH 7.4
- 1M MgCl2
- 10x Phosphate Buffered Saline (PBS)
- 20x Phosphate Buffered Saline (PBS)
- PBT
- Proteinase K(PK), 10 mg/ml
- PTw
- 4M NaCl, 1 liter
- SDS 10%
- TAE 50X
- 1M Tris pH 9.5
Additional Solutions/Reagents: