Alpha-tubulin - For labeling microtubules
Antibody name: DM1A
Raised in: Mouse
Subtype: IgG
Antigen: Microtubules from chick embryo brain
Fixatives: Works well in MEMFA or Dent's
Sensitive to bleaching: Yes
Functions post in situ?: Yes
Immunoprecipitation/Western blotting:
Available as: Ascites
Available from: Sigma (cat#T9026)
Optimized conditions: We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room tempp for four hours.
Other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse