E-cadherin - cdh1

Antigen: E-cadherin

Epitope: 140 kDa xenopus E-cadherin

Host Species/Isotype: mouse monoclonal IgG2b

Antigen Species: xenopus

Applications: IF

Antibody Name: 5D3 [1]

Supplier: Developmental Systems Hybridoma Bank

Comments: IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300

Contributor: Zorn Lab and V Ponferrada

Additional Information: MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work

Reference(s):

applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry