Anti-FLAG M2 monoclonal antibody

Antigen: FLAG epitope

Epitope: aa DYKDDDDK

Host Species/Isotype: mouse monoclonal, IgG1

Antigen Species: synthetic peptide

Applications: IP, IF, WB

Antibody Name: F3165 [1]

Supplier: Sigma

Comments: IP: use 2 ug, IF: use at 1:1000, WB use at 1:5000 to 1:10,000

Contributor: Zorn Lab and V Ponferrada

Additional Information: IF: MEMFA, 10% TCA fixatives work

Reference(s):

applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry