High-salt solution for egg laying (Zorn lab/Ron Laskey)

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Protocol submitted by VGP


RAL’s Ca++-free High Salt Barth: Egg Laying (8X)


___________________(1x solution)___________________________amounts to make 8X stock__________

_____________________________________________2 litres___________4 litres___________8 liters_________

NaCl_________________110 mM___________________102.85g___________205.71g___________308.55g

Tris base______________15 mM____________________29.075g___________58.15g____________87.23g

KCl____________________2 mM____________________2.385g_____________4.77g____________7.2g

Na2HPO4 (anhydrous)___0.5 mM____________________1.36g______________2.72g____________4.1g

NaHCO3________________2 mM____________________2.69g______________5.38g____________8.07g

Adjust pH to 7.6 with glacial acetic acid (approx. 25 mls for 4 litres)

MgSO4.7H2O____________1mM____________________7.89g______________3.945g___________11.84g


Make 8X stock, dilute to 1x solution in dH2O just before use. Maximum recommended storage: 8 weeks at 4oC.

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